Growth of subtropical ECM fungi with different nitrogen sources using a new floating culture technique

Sangtiean, T. and Schmidt, S. (2002) Growth of subtropical ECM fungi with different nitrogen sources using a new floating culture technique. Mycological Research, 106 1: 74-85. doi:10.1017/S0953756201005226

Author Sangtiean, T.
Schmidt, S.
Title Growth of subtropical ECM fungi with different nitrogen sources using a new floating culture technique
Journal name Mycological Research   Check publisher's open access policy
ISSN 0953-7562
Publication date 2002-01-01
Year available 2002
Sub-type Article (original research)
DOI 10.1017/S0953756201005226
Open Access Status Not yet assessed
Volume 106
Issue 1
Start page 74
End page 85
Total pages 12
Place of publication New York, USA
Publisher Cambridge Univ Press
Language eng
Subject C1
270305 Mycology
770703 Living resources (flora and fauna)
Abstract Eight species of ectomycorrhizal (ECM) fungi in the genera Amanita. Gymnoboletus, Lactarius, and Russula were isolated from subtropical plant communities in eastem Australia. Two species were isolated from each of rainforest, Nothofagus forest, Eucalyptus forest, and Eucalyptus dominated wallum (heath) forest. These communities differ strongly in their soluble soil nitrogen (N) composition. The ability of the fungi to use inorganic (nitrate, ammonium) and organic (amide, peptide, protein) nitrogen sources was determined. As the fungi did not grow in liquid culture, a 'floating culture' technique was devised that allows hyphal growth on a screen floating on liquid medium. With some exceptions, fungal biomass production in floating culture closely reflected fungal growth on solid media assessed by total colony glucosamine content. Most isolates grown in floating culture had similar glucosamine concentrations on all N sources, with isolate specific concentrations ranging from 6 to 12 mug glucosamine g(-1) DW. However, Russula spp. had up to 1.7-fold higher glucosamine concentrations when growing with glutamine or ammonium compared to nitrate, glutathione or protein. Floating cultures supplied with 0.5, 1.5. 4.5, or 10 mm N mostly produced greatest biomass with 4.5 mM N. In vitro nitrate reductase activity (NRA) ranged from very low (0.03 mumol NO2- g(-1) fw h(-1)) in Russula sp. (wallum) to high (2.16 mumol NO2- g(-1) fw h(-1)) in Gymnoboletus sp. (rainforest) and mirrored the fungi's ability to use nitrate as a N source. All Russula spp. (wallum, Nothofagus and Eucalyptus forests), Lactarills sp, (rainforest) and.4manita sp. (wallum) utilized ammonium and glutamine but had little ability to use other N sources. In contrast,Amanita species (Nothofagus and Eucalyptus forests) grew on all N sources but produced most biomass with ammonium and glutamine. Only Gymnoboletus sp. (rainforest) showed similar growth with nitrate and ammonium as N sources. Fungal N source use was not associated with taxonomic groups, but is discussed in the context of soil N sources in the different habitats.
Keyword Mycology
Ectomycorrhizal Fungi
Mycorrhizal Fungi
Pure Culture
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
Ecology Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 20 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 19 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 03:27:17 EST