Saccharomyces cerevisiae is permissive for replication of bovine papillomavirus type 1

Zhao, Kong-Nan and Frazer, Ian H. (2002) Saccharomyces cerevisiae is permissive for replication of bovine papillomavirus type 1. Journal of Virology, 76 23: 12265-12273. doi:10.1128/JVI.76.23.12265-12273.2002


Author Zhao, Kong-Nan
Frazer, Ian H.
Title Saccharomyces cerevisiae is permissive for replication of bovine papillomavirus type 1
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2002-12-01
Year available 2002
Sub-type Article (original research)
DOI 10.1128/JVI.76.23.12265-12273.2002
Open Access Status DOI
Volume 76
Issue 23
Start page 12265
End page 12273
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Subject C1
320402 Medical Virology
730101 Infectious diseases
Abstract We recently demonstrated that Saccharomyces cerevisiae protoplasts can take up bovine papillomavirus type 1 (BPV1) virions and that viral episomal DNA is replicated after uptake. Here we demonstrate that BPV virus-like particles are assembled in infected S. cerevisiae cultures from newly synthesized capsid proteins and also package newly synthesized DNA, including full-length and truncated viral DNA and S. cerevisiae-derived DNA. Virus particles prepared in S. cerevisiae are able to convey packaged DNA to Cos1 cells and to transform C127 cells. Infectivity was blocked by antisera to BPV1 L1 but not antisera to BPV1 E4. We conclude that S. cerevisiae is permissive for the replication of BPV1 virus.
Keyword Virology
Virus-like Particles
Major Capsid Protein
Keratinocyte Cell-line
Neutralizing Antibodies
Candida-albicans
Alpha-6 Integrin
Life-cycle
Viral-dna
Expression
L1
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
UQ Diamantina Institute Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 20 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 22 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 03:02:56 EST