SOX18 directly interacts with MEF2C in endothelial cells

Hosking, B. M., Wang, S. C. M., Chen, S. L., Penning, S., Koopman, P. and Muscat, G. E. O. (2001) SOX18 directly interacts with MEF2C in endothelial cells. Biochemical And Biophysical Research Communications, 287 2: 493-500. doi:10.1006/bbrc.2001.5589


Author Hosking, B. M.
Wang, S. C. M.
Chen, S. L.
Penning, S.
Koopman, P.
Muscat, G. E. O.
Title SOX18 directly interacts with MEF2C in endothelial cells
Journal name Biochemical And Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
Publication date 2001-01-01
Sub-type Article (original research)
DOI 10.1006/bbrc.2001.5589
Volume 287
Issue 2
Start page 493
End page 500
Total pages 8
Place of publication San Diego
Publisher Academic Press
Collection year 2001
Language eng
Subject C1
Abstract Recently, we demonstrated that mutations in the Sry-related HMG box gene Sox18 underlie vascular and hair follicle defects in the mouse allelic mutants ragged (Ra) and RaJ. Ra mice display numerous anomalies in the homozygote including, oedema, peritoneal secretions, and are almost completely naked. Sox18 and the MADS box transcription factor, Mef2C, are expressed in developing endothelial cells. Null mutants in Sox18 and Mef2c display overlapping phenotypic abnormalities, hence, we investigated the relationship between these two DNA binding proteins. We report here the direct interaction between MEF2C and SOX18 proteins, and establish that these proteins are coexpressed in vivo in endothelial cell nuclei. MEF2C expression potentiates SOX18-mediated transcription in vivo and regulates the function of the SOX18 activation domain. Interestingly, MEF2C fails to interact or co-activate transcription with the Ra or RaJ mutant SOX18 proteins. These results suggest that MEF2C and SOX18 may be important partners directing the transcriptional regulation of vascular development. (C) 2001 Academic Press.
Keyword Biochemistry & Molecular Biology
Biophysics
Sox18
Mef2c
Transcription
Protein-protein Interaction
Vasculogenesis
Endothelial
Ragged
Transcription Factor Mef2c
Mouse Embryogenesis
Gene-expression
Growth-factor
Mads-box
Transactivation
Proteins
Mechanisms
Sequence
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Wed, 15 Aug 2007, 02:59:15 EST