Apoptotic deletion of Th cells specific for the 19-kDa carboxyl-terminal fragment of merozoite surface protein 1 during malaria infection

Wipasa, Jiraprapa, Xu, Huji, Stowers, Anthony and Good, Michael F. (2001) Apoptotic deletion of Th cells specific for the 19-kDa carboxyl-terminal fragment of merozoite surface protein 1 during malaria infection. Journal of Immunology, 167 7: 3903-3909.

Author Wipasa, Jiraprapa
Xu, Huji
Stowers, Anthony
Good, Michael F.
Title Apoptotic deletion of Th cells specific for the 19-kDa carboxyl-terminal fragment of merozoite surface protein 1 during malaria infection
Journal name Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
Publication date 2001-01-01
Sub-type Article (original research)
Open Access Status Not Open Access
Volume 167
Issue 7
Start page 3903
End page 3909
Total pages 7
Place of publication Rockville Pike, USA
Publisher American Association of Immunologists
Language eng
Subject CX
730102 Immune system and allergy
320405 Medical Parasitology
Abstract Immunity induced by the 19-kDa fragment of merozoite surface protein 1 is dependent on CD4(+) Th cells. However, we found that adoptively transferred CFSE-labeled Th cells specific for an epitope on Plasmodium yoelii 19-kDa fragment of merozoite surface protein 1 (peptide (p)24), but not OVA-specific T cells, were deleted as a result of P. yoelii infection. As a result of infection, spleen cells recovered from infected p24-specific T cell-transfused mice demonstrated reduced response to specific Ag. A higher percentage of CFSE-labeled p24-specific T cells stained positive with annexin and anti-active caspase-3 in infected compared with uninfected mice, suggesting that apoptosis contributed to deletion of p24-specific T cells during infection. Apoptosis correlated with increased percentages of p24-specific T cells that stained positive for Fas from infected mice, suggesting that P. yoelii-induced apoptosis is, at least in part, mediated by Fas. However, bystander cells of other specificities also showed increased Fas expression during infection, suggesting that Fas expression alone is not sufficient for apoptosis. These data have implications for the development of immunity in the face of endemic parasite exposure.
Keyword Immunology
Fas-mediated Apoptosis
Plasmodium-falciparum
Protective Immunity
Mononuclear-cells
Interferon-gamma
Cd95 Fas/apo-1
Tnf-alpha
Ifn-gamma
In-vivo
Death
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 02:49:40 EST