Isolation of a novel G-protein gamma-subunit from Arabidopsis thaliana and its interaction with G beta

Mason, Michael G. and Botella, José R. (2001) Isolation of a novel G-protein gamma-subunit from Arabidopsis thaliana and its interaction with G beta. Biochimica et biophysica acta (BBA). Gene structure and expression, 1520 2: 147-153. doi:10.1016/S0167-4781(01)00262-7


Author Mason, Michael G.
Botella, José R.
Title Isolation of a novel G-protein gamma-subunit from Arabidopsis thaliana and its interaction with G beta
Journal name Biochimica et biophysica acta (BBA). Gene structure and expression   Check publisher's open access policy
ISSN 0167-4781
Publication date 2001-08-30
Year available 2001
Sub-type Article (original research)
DOI 10.1016/S0167-4781(01)00262-7
Open Access Status Not yet assessed
Volume 1520
Issue 2
Start page 147
End page 153
Total pages 7
Place of publication Amsterdam
Publisher Elsevier Biomedical Press
Language eng
Subject C1
270202 Genome Structure
780105 Biological sciences
Abstract There is increasing evidence that heterotrimeric G-proteins (G-proteins) are involved in many plant processes including phytohormone response, pathogen defence and stomatal control. In animal systems, each of the three G-protein subunits belong to large multigene families; however, few subunits have been isolated from plants. Here we report the cloning of a second plant G-protein γ-subunit (AGG2) from Arabidopsis thaliana. The predicted AGG2 protein sequence shows 48% identity to the first identified Arabidopsis Gγ-subunit, AGG1. Furthermore, AGG2 contains all of the conserved characteristics of γ-subunits including a small size (100 amino acids, 11.1 kDa), C-terminal CAAX box and a N-terminal α-helix region capable of forming a coiled-coil interaction with the β-subunit. A strong interaction between AGG2 and both the tobacco (TGB1) and Arabidopsis (AGB1) β-subunits was observed in vivo using the yeast two-hybrid system. The strong association between AGG2 and AGB1 was confirmed in vitro. Southern and Northern analyses showed that AGG2 is a single copy gene in Arabidopsis producing two transcripts that are present in all tissues tested. The isolation of a second γ-subunit from A. thaliana indicates that plant G-proteins, like their mammalian counterparts, may form different heterotrimer combinations that presumably regulate multiple signal transduction pathways.
Keyword G-Protein
Yeast two-hybrid
γ-Subunit
Coiled coil
Arabidopsis thaliana
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Wed, 15 Aug 2007, 02:48:56 EST