Spatial distribution and developmental appearance of postjunctional P2X(1) receptors on smooth muscle cells of the mouse vas deferens

Liang, SX, Motin, L, Moussa, CEH, Lavidis, NA and Phillips, WD (2001) Spatial distribution and developmental appearance of postjunctional P2X(1) receptors on smooth muscle cells of the mouse vas deferens. Synapse, 42 1: 1-11. doi:10.1002/syn.1094


Author Liang, SX
Motin, L
Moussa, CEH
Lavidis, NA
Phillips, WD
Title Spatial distribution and developmental appearance of postjunctional P2X(1) receptors on smooth muscle cells of the mouse vas deferens
Journal name Synapse   Check publisher's open access policy
ISSN 0887-4476
Publication date 2001-01-01
Sub-type Article (original research)
DOI 10.1002/syn.1094
Open Access Status Not yet assessed
Volume 42
Issue 1
Start page 1
End page 11
Total pages 11
Editor John E Johnson Jr
Place of publication USA
Publisher John Wiley & Sons Inc
Language eng
Subject C1
320701 Autonomic Nervous System
730104 Nervous system and disorders
Abstract P2X(1)-type purinoceptors, have been shown to mediate fast transmission between sympathetic varicosities and smooth muscle cells in the mouse vas deferens but the spatial organization of these receptors on the smooth muscle cells remains inconclusive. Voltage clamp techniques were used to estimate the amplitudes of spontaneous excitatory junction currents (SEJCs) in cells of the vas deferens longitudinal smooth muscle layer. These currents involved the activation of about 6% of the P2X-type channels present on the cell, as compared to whole cell currents produced when isolated smooth muscle cells were exposed to maximal concentrations of either ATP or alpha,beta -MeATP. Immunofluorescence staining of the vas deferens with antibodies against P2X(1) receptor showed a diffuse, grainy distribution over the entire membrane of each smooth muscle cell. Anti-P2X(1) staining was not markedly clustered beneath anti-SV2-stained sympathetic varicosities. Similar results were obtained for cells in the urinary bladder. During development, P2X(1) mRNA was detected as early as embryonic day 15 (E15). Increasing intensities of diffuse immunostaining for P2X(1) were observed in the walls of the bladder, tail artery, and aorta from E15 until 6 weeks postnatal. The vas deferens showed increasing intensities of diffuse staining of its smooth muscle layers between 2 and 6 weeks postnatal, consistent with the time-course of development of fast purinergic transmission described previously. Together, the results suggest that the response of smooth muscle of the vas deferens to ATP released from sympathetic varicosities relies on rapidly desensitizing P2X(1) receptors, distributed diffusely across the smooth muscle cell surface. Synapse 42:1-11, 2001. (C) 2001 Wiley-Liss, Inc.
Keyword Neurosciences
Receptors
Purinergic P2
Muscle
Smooth
Vascular
Neuromuscular Junction
Bladder
Sympathetic Fibers
Postganglionic
Gated Ion Channels
Sympathetic Varicosities
Neuromuscular-junctions
Acetylcholine-receptor
Sensory Neurons
Urinary-bladder
P-2x Receptor
Atp
Rat
Purinoceptor
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Wed, 15 Aug 2007, 02:47:11 EST