De novo generation and characterization of new Zika virus isolate using sequence data from a microcephaly case

Setoh, Yin Xiang, Prow, Natalie A., Peng, Nias, Hugo, Leon E., Devine, Gregor, Hazlewood, Jessamine E., Suhrbier, Andreas and Khromykh, Alexander A. (2017) De novo generation and characterization of new Zika virus isolate using sequence data from a microcephaly case. mSphere, 2 3: 1-11. doi:10.1128/mSphereDirect.00190-17


Author Setoh, Yin Xiang
Prow, Natalie A.
Peng, Nias
Hugo, Leon E.
Devine, Gregor
Hazlewood, Jessamine E.
Suhrbier, Andreas
Khromykh, Alexander A.
Title De novo generation and characterization of new Zika virus isolate using sequence data from a microcephaly case
Formatted title
De novo generation and characterization of new Zika virus isolate using sequence data from a microcephaly case
Journal name mSphere   Check publisher's open access policy
ISSN 2379-5042
Publication date 2017-05-17
Year available 2017
Sub-type Article (original research)
DOI 10.1128/mSphereDirect.00190-17
Open Access Status DOI
Volume 2
Issue 3
Start page 1
End page 11
Total pages 11
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Zika virus (ZIKV) has recently emerged and is the etiological agent of congenital Zika syndrome (CZS), a spectrum of congenital abnormalities arising from neural tissue infections in utero. Herein, we describe the de novo generation of a new ZIKV isolate, ZIKV(Natal), using a modified circular polymerase extension reaction protocol and sequence data obtained from a ZIKV-infected fetus with microcephaly. ZIKVNatal thus has no laboratory passage history and is unequivocally associated with CZS. ZIKVNatal could be used to establish a fetal brain infection model in IFNAR(-/-) mice (including intrauterine growth restriction) without causing symptomatic infections in dams. ZIKVNatal was also able to be transmitted by Aedes aegypti mosquitoes. ZIKVNatal thus retains key aspects of circulating pathogenic ZIKVs and illustrates a novel methodology for obtaining an authentic functional viral isolate by using data from deep sequencing of infected tissues.
Keyword Zika virus
Flavivirus
Infectious DNA
Mouse model
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Article number e00190-17

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 26 May 2017, 10:41:37 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences