Characterization of a novel gene, STAG1/PMEPA1, upregulated in renal cell carcinoma and other solid tumors

Rae, FK, Hooper, JD, Nicol, DL and Clements, JA (2001) Characterization of a novel gene, STAG1/PMEPA1, upregulated in renal cell carcinoma and other solid tumors. Molecular Carcinogenesis, 32 1: 44-53. doi:10.1002/mc.1063

Author Rae, FK
Hooper, JD
Nicol, DL
Clements, JA
Title Characterization of a novel gene, STAG1/PMEPA1, upregulated in renal cell carcinoma and other solid tumors
Journal name Molecular Carcinogenesis   Check publisher's open access policy
ISSN 0899-1987
Publication date 2001-01-01
Sub-type Article (original research)
DOI 10.1002/mc.1063
Volume 32
Issue 1
Start page 44
End page 53
Total pages 10
Place of publication New York
Publisher Wiley-Liss
Language eng
Subject C1
321099 Clinical Sciences not elsewhere classified
730108 Cancer and related disorders
Abstract Using differential display-polymerase chain reaction, we identified a novel gene sequence, designated solid tumor-associated gene 1 (STAG1), that is upregulated in renal cell carcinoma (RCC). The full-length cDNA (4839 bp) encompassed the recently reported androgen-regulated prostatic cDNA PMEPA1 and so we refer to this gene as STAG1/PMEPA1, Two STAG1/PMEPA1 mRNA transcripts of approximately 2.7 an 5 kb, with identical coding regions but variant 3' untranslated regions, were predominantly expressed in normal prostate tissue and at lower levels in the ovary. The expression of this gene was upregulated in 87% of RCC samples and also was upregulated in stomach and rectal adenocarcinomas. In contrast, STAG1/PMEPA1 expression was barely detectable in leukemia and lymphoma samples, Analysis of expressed sequence tag databases showed that STAG1/PMEPA1 also was expressed in pancreatic, endometrial, and prostatic adenocarcinomas. The STAG1/PMEPA1 cDNA encodes a 287-amino-acid protein containing a putative transmembrane domain and motifs that suggest that it may bind src homology 3- and tryptophan tryptophan domain-containing proteins. This protein shows 67% identity to the protein encoded by the chromosome 18 open reading frame 1 gene. Translation of STAG1/PMEPA1 mRNA in vitro showed two products of 36 and 39 kDa, respectively, suggesting that translation may initiate at more than one site. Comparison to genomic clones showed that STAG1/PMEPA1 was located on chromosome 20q13 between microsatellite markers D20S183 and D20S173 and spanned four exons and three introns. The upregulation of this gene in several solid tumors indicated that it may play an important role in tumorigenesis. (C) 2001 Wiley-Liss, Inc.
Keyword Biochemistry & Molecular Biology
Differential Display
Polymerase Chain Reaction
Gene Expression
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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Created: Wed, 15 Aug 2007, 02:43:08 EST