Imaging brain deoxyglucose uptake and metabolism by glucoCEST MRI

Nasrallah, Fatima A., Pages, Guilhem, Kuchel, Philip W., Golay, Xavier and Chuang, Kai-Hsiang (2013) Imaging brain deoxyglucose uptake and metabolism by glucoCEST MRI. Journal of Cerebral Blood Flow and Metabolism, 33 8: 1270-1278. doi:10.1038/jcbfm.2013.79

Author Nasrallah, Fatima A.
Pages, Guilhem
Kuchel, Philip W.
Golay, Xavier
Chuang, Kai-Hsiang
Title Imaging brain deoxyglucose uptake and metabolism by glucoCEST MRI
Journal name Journal of Cerebral Blood Flow and Metabolism   Check publisher's open access policy
ISSN 0271-678X
Publication date 2013-08-01
Year available 2013
Sub-type Article (original research)
DOI 10.1038/jcbfm.2013.79
Open Access Status Not yet assessed
Volume 33
Issue 8
Start page 1270
End page 1278
Total pages 9
Place of publication London, United Kingdom
Publisher Sage Publications
Language eng
Formatted abstract
2-Deoxy-D-glucose (2DG) is a known surrogate molecule that is useful for inferring glucose uptake and metabolism. Although 13 C-labeled 2DG can be detected by nuclear magnetic resonance (NMR), its low sensitivity for detection prohibits imaging to be performed. Using chemical exchange saturation transfer (CEST) as a signal-amplification mechanism, 2DG and the phosphorylated 2DG-6-phosphate (2DG6P) can be indirectly detected in 1H magnetic resonance imaging (MRI). We showed that the CEST signal changed with 2DG concentration, and was reduced by suppressing cerebral metabolism with increased general anesthetic. The signal changes were not affected by cerebral or plasma pH, and were not correlated with altered cerebral blood flow as demonstrated by hypercapnia; neither were they related to the extracellular glucose amounts as compared with injection of D-and L-glucose. In vivo 31 P NMR revealed similar changes in 2DG6P concentration, suggesting that the CEST signal reflected the rate of glucose assimilation. This method provides a new way to use widely available MRI techniques to image deoxyglucose/glucose uptake and metabolism in vivo without the need for isotopic labeling of the molecules.
Keyword 2-deoxyglucose
Magnetic resonance imaging
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
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