DAF yields a cloned marker linked to the soybean (Glycine max) supernodulation nts-1 locus

Men, A. E. and Gresshoff, P. M. (2001) DAF yields a cloned marker linked to the soybean (Glycine max) supernodulation nts-1 locus. Journal of Plant Physiology, 158 8: 999-1006. doi:10.1078/0176-1617-00303


Author Men, A. E.
Gresshoff, P. M.
Title DAF yields a cloned marker linked to the soybean (Glycine max) supernodulation nts-1 locus
Journal name Journal of Plant Physiology   Check publisher's open access policy
ISSN 0176-1617
Publication date 2001-01-01
Sub-type Article (original research)
DOI 10.1078/0176-1617-00303
Open Access Status Not Open Access
Volume 158
Issue 8
Start page 999
End page 1006
Total pages 8
Editor C. Wilhelm
Place of publication Jena, Germany
Publisher Urban & Fischer Verlag
Language eng
Subject C1
270202 Genome Structure
620108 Grain legumes
Abstract We report a further characterization of the genomic region containing the soybean supernodulation gene NTS-1. We performed a search for new markers linked to NTS-1 by combining DNA amplification fingerprinting (DAF) and bulked segregant analysis (BSA). The search resulted in one cloned polymorphism (B44-456) linked in trans, 8.5cM from the locus. Southern hybridization showed duplication of the B44-456 sequence in the soybean genome. Additionally, a DNA database search revealed one Arabidopsis thaliana genomic clone from chromosome I possessing 62% homology to the B44-456 marker. A relatively low number of polymorphisms were identified by several PCR marker technologies for this soybean genomic region, providing an additional support for its highly conserved and/or duplicated organization.
Keyword Plant Sciences
Bulked Segregant Analysis
Daf
Soybean
Supernodulation
Symbiosis
Disease-resistance
Oligonucleotide Primers
Polyacrylamide Gels
Genome Duplication
Linkage Map
Gene
Dna
Cloning
Amplification
Tomato
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Integrative Legume Research Publications
 
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Created: Wed, 15 Aug 2007, 02:36:04 EST