A comparative investigation of Bombyx mori silk fibroin hydrogels generated by chemical and enzymatic cross-linking

Chirila, Traian V., Suzuki, Shuko and Papolla, Chloe (2017) A comparative investigation of Bombyx mori silk fibroin hydrogels generated by chemical and enzymatic cross-linking. Biotechnology and Applied Biochemistry, 64 6: 771-781. doi:10.1002/bab.1552


Author Chirila, Traian V.
Suzuki, Shuko
Papolla, Chloe
Title A comparative investigation of Bombyx mori silk fibroin hydrogels generated by chemical and enzymatic cross-linking
Journal name Biotechnology and Applied Biochemistry   Check publisher's open access policy
ISSN 1470-8744
0885-4513
Publication date 2017-04-27
Sub-type Article (original research)
DOI 10.1002/bab.1552
Open Access Status Not yet assessed
Volume 64
Issue 6
Start page 771
End page 781
Total pages 11
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Subject 1305 Biotechnology
1502 Bioengineering
1313 Molecular Medicine
2204 Biomedical Engineering
2402 Applied Microbiology and Biotechnology
3002 Drug Discovery
1508 Process Chemistry and Technology
Abstract Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrogels that present certain advantages when employed as biomaterials, especially if they are cross-linked. The subject of this study was the self-cross-linking of silk fibroin through a process induced by the enzyme horseradish peroxidase (HRP) in the presence of hydrogen peroxide, a method only recently proposed and scarcely reported. The hydrogels were prepared either by physical cross-linking, by cross-linking with a natural compound (genipin), or by enzymatic cross-linking. The products were comparatively characterized in regard to their synthesis and background chemical aspects, physical and optical properties, mechanical properties, secondary structure, swelling/deswelling behavior, enzymatic degradation, and compatibility as substrates for cell adhesion and proliferation. The study confirmed the advantages of the HRP-induced cross-linking, which included considerably shorter gelation times, enhanced elasticity of the resulting hydrogels, and improved cytocompatibility. Discrepancies between certain results of this investigation and those reported previously were discussed in detail.
Keyword Biomaterials
Cell cultivation
Enzymatic reactions
Genipin
Horseradish peroxidase
Silk fibroin
Tissue engineering
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID APP1080302
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Australian Institute for Bioengineering and Nanotechnology Publications
 
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