The effects of interleukin-10 depletion in vivo on the immune response to Porphyromonas gingivalis in a murine model

Herminajeng, E., Sosroseno, W., Bird, P. S. and Seymour, G. J. (2001) The effects of interleukin-10 depletion in vivo on the immune response to Porphyromonas gingivalis in a murine model. Journal of Periodontology, 72 11: 1527-1534. doi:10.1902/jop.2001.72.11.1527

Author Herminajeng, E.
Sosroseno, W.
Bird, P. S.
Seymour, G. J.
Title The effects of interleukin-10 depletion in vivo on the immune response to Porphyromonas gingivalis in a murine model
Journal name Journal of Periodontology   Check publisher's open access policy
ISSN 0022-3492
Publication date 2001-01-01
Sub-type Article (original research)
DOI 10.1902/jop.2001.72.11.1527
Open Access Status
Volume 72
Issue 11
Start page 1527
End page 1534
Total pages 8
Editor Robert Genco
Place of publication Chicago
Publisher American Academy of Periodontology
Language eng
Subject C1
320899 Dentistry not elsewhere classified
730112 Oro-dental and disorders
1105 Dentistry
Abstract Background: The immune response to Porphyromonas gingivalis in the mouse abscess model is known to be dependent upon CD4 T-cell activation and the regulatory role of cytokines. The role of interleukin-10 (IL-10) in this mouse model was examined in vivo. Methods: One-week-old, female BALB/c mice were divided into 4 groups. Groups 1 and 2 were given intraperitoneal (ip) injections of phosphate buffered saline (PBS) weekly for 5 weeks. Group 3 was given an ip injection of rat immunoglobulin. Group 4 was injected with rat anti-IL-10 antibodies. At week 6, group 1 was sham-immunized with PBS, and groups 2, 3, and 4 were injected with P gingivalis lipopolysaccharide (Pg-LPS) weekly for 2 weeks. One week after the final immunization, delayed-type hypersensitivity (DTH) was assessed by footpad swelling to Pg-LPS. The level of serum antibodies to Pg-LPS and IFN-gamma (IFN-gamma) was determined by enzyme-linked immunosorbent assay. Dorsal abscess formation induced by the injection of viable P gingivalis was examined daily for 30 days. Results: The footpad swelling of the anti-IL-10-treated group (group 4) was significantly higher than that of groups 1 to 3. Similarly, the serum IFN-gamma level in group 4 was much higher than that of the other experimental groups. There was no significant difference in serum IgG antibodies to Pg-LPS in any of the experimental groups. However, the level of IgM antibodies in group 4 mice was significantly lower than that in groups 2 and 3. In addition, serum IgG1 was suppressed in group 4 mice, while IgG2a antibodies were raised. However, there was no difference observed between the levels of IgG2b and IgG3 antibodies in any group of mice. The lesions in sham-immunized mice (group 1) persisted for 30 days, and those in group 2 and 3 were undetected by day 18 and 20, respectively. In sharp contrast, lesions in group 4 had healed completely by day 13. Conclusions: This study has shown that IL-10 depletion in vivo in P gingivalis LPS-induced immune response in mice led to an elevated DTH response, an increase in serum IFN-gamma levels, and raised levels of IgG and IgG2a antibodies. Treatment with anti-IL-10 antibodies resulted in suppressed IgG I and IgM responses and a more rapid healing of abscesses than in non-IL-10-depleted mice. These results suggest that IL-10 depletion in Pg-LPS-induced immune response in mice may lead to a Th1-like immune response and provide strong protection against a subsequent challenge with live P gingivalis in an abscess model.
Keyword Dentistry, Oral Surgery & Medicine
Porphyromonas Gingivalis
Animal Studies
Disease Models
Immune Response
Inflammatory Periodontal-disease
Anticollagen Antibody-production
Cytokine Profiles
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 15 Aug 2007, 02:19:21 EST