Novel chitinase gene LOC_Os11g47510 from indica rice tetep provides enhanced resistance against sheath blight pathogen Rhizoctonia solani in rice

Richa, Kamboj, Tiwari, Ila M., Devanna, B. N., Botella, Jose R., Sharma, Vinay and Sharma, Tilak R. (2017) Novel chitinase gene LOC_Os11g47510 from indica rice tetep provides enhanced resistance against sheath blight pathogen Rhizoctonia solani in rice. Frontiers in Plant Science, 8 596. doi:10.3389/fpls.2017.00596


Author Richa, Kamboj
Tiwari, Ila M.
Devanna, B. N.
Botella, Jose R.
Sharma, Vinay
Sharma, Tilak R.
Title Novel chitinase gene LOC_Os11g47510 from indica rice tetep provides enhanced resistance against sheath blight pathogen Rhizoctonia solani in rice
Formatted title
Novel chitinase gene LOC_Os11g47510 from indica rice tetep provides enhanced resistance against sheath blight pathogen Rhizoctonia solani in rice
Journal name Frontiers in Plant Science   Check publisher's open access policy
ISSN 1664-462X
Publication date 2017-04-25
Year available 2017
Sub-type Article (original research)
DOI 10.3389/fpls.2017.00596
Open Access Status DOI
Volume 8
Start page 596
Total pages 10
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Language eng
Subject 1110 Plant Science
Abstract Sheath blight disease (ShB), caused by the fungus Rhizoctonia solani Kuhn, is one of the most destructive diseases of rice (Oryza sativa L.), causing substantial yield loss in rice. In the present study, a novel rice chitinase gene, LOC_Os11g47510 was cloned from QTL region of R. solani tolerant rice line Tetep and used for functional validation by genetic transformation of ShB susceptible japonica rice line Taipei 309 (TP309). The transformants were characterized using molecular and functional approaches. Molecular analysis by PCR using a set of primers specific to CaMv 35S promoter, chitinase and HptII genes confirmed the presence of transgene in transgenic plants which was further validated by Southern hybridization. Further, qRT-PCR analysis of transgenic plants showed good correlation between transgene expression and the level of sheath blight resistance among transformants. Functional complementation assays confirmed the effectiveness of the chitinase mediated resistance in all the transgenic TP309 plants with varying levels of enhanced resistance against R. solani. Therefore, the novel chitinase gene cloned and characterized in the present study from the QTL region of rice will be of significant use in molecular plant breeding program for developing sheath blight resistance in rice.
Formatted abstract
Sheath blight disease (ShB), caused by the fungus Rhizoctonia solani Kühn, is one of the most destructive diseases of rice (Oryza sativa L.), causing substantial yield loss in rice. In the present study, a novel rice chitinase gene, LOC_Os11g47510 was cloned from QTL region of R. solani tolerant rice line Tetep and used for functional validation by genetic transformation of ShB susceptible japonica rice line Taipei 309 (TP309). The transformants were characterized using molecular and functional approaches. Molecular analysis by PCR using a set of primers specific to CaMv 35S promoter, chitinase and HptII genes confirmed the presence of transgene in transgenic plants which was further validated by Southern hybridization. Further, qRT-PCR analysis of transgenic plants showed good correlation between transgene expression and the level of sheath blight resistance among transformants. Functional complementation assays confirmed the effectiveness of the chitinase mediated resistance in all the transgenic TP309 plants with varying levels of enhanced resistance against R. solani. Therefore, the novel chitinase gene cloned and characterized in the present study from the QTL region of rice will be of significant use in molecular plant breeding program for developing sheath blight resistance in rice.
Keyword Chitinase
Rice
Rhizoctonia solani
Sheath blight
Qtl
Tetep
Transgenic
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID BT/Indo-Qld/ 01/09/2010
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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