Wolbachia-mediated sperm modification is dependent on the host genotype in Drosophila

McGraw, E. A., Merritt, D. J., Droller, J. N. and O'Neill, S. L. (2001) Wolbachia-mediated sperm modification is dependent on the host genotype in Drosophila. Proceedings of the Royal Society of London Series B-Containing Papers of a Biological Character, 268 1485: 2565-2570. doi:10.1098/rspb.2001.1839


Author McGraw, E. A.
Merritt, D. J.
Droller, J. N.
O'Neill, S. L.
Title Wolbachia-mediated sperm modification is dependent on the host genotype in Drosophila
Journal name Proceedings of the Royal Society of London Series B-Containing Papers of a Biological Character   Check publisher's open access policy
ISSN 0962-8452
Publication date 2001-12-22
Year available 2001
Sub-type Article (original research)
DOI 10.1098/rspb.2001.1839
Open Access Status Not yet assessed
Volume 268
Issue 1485
Start page 2565
End page 2570
Total pages 6
Editor R. M. Alexander
Place of publication London
Publisher The Royal Society of London
Language eng
Subject C1
270304 Infectious Agents
780105 Biological sciences
270299 Genetics not elsewhere classified
770804 Control of pests and exotic species
270000 Biological Sciences
270300 Microbiology
270200 Genetics
06 Biological Sciences
Abstract Estimates of Wolbachia density in the eggs, testes and whole flies of drosophilid hosts have been unable to predict the lack of cytoplasmic incompatibility (CI) expression in so-called mod(-) variants. Consequently, the working hypothesis has been that CI expression, although related to Wolbachia density, is also governed by unknown factors that are influenced by both host and bacterial genomes. Here, we compare the behaviour of the mod(-) over-replicating Wolbachia popcorn strain in its native Drosophila melanogaster host to the same strain transinfected into a novel host, namely Drosophila simulans. We report that (i) the popcorn strain is a close relative of other D. melanogaster infections, (ii) the mod(-) status of popcorn in D. melanogaster appears to result from its inability to colonize sperm bundles, (iii) popcorn is present in the bundles in D. simulans and induces strong CI expression, which demonstrates that the bacterial strain does not lack the genetic machinery for inducing CI and that there is host-species-specific control over Wolbachia tissue tropism, and (iv) infection of sperm bundles by the mod(-) D. simulans wCof strain indicates that there are several independent routes by which a strain can be a CI non-expressor.
Keyword Wolbachia
cytoplasmic incompatibility
popcorn
sperm modi¬Ęcation
Sperm Modification
Simulans
Melanogaster
Endosymbionts
Populations
Expression
Infection
Evolution
Sequences
Symbiont
Q-Index Code C1
Grant ID AI40620
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Wed, 15 Aug 2007, 02:17:20 EST