Gag-Pol supplied in trans is efficiently packaged and supports viral function in human immunodeficiency virus type 1

Hill, M. K., Hooker, C. W., Harrich, D., Crowe, S. M. and Mak, J. (2001) Gag-Pol supplied in trans is efficiently packaged and supports viral function in human immunodeficiency virus type 1. Journal of Virology, 75 15: 6835-6840. doi:10.1128/JVI.75.15.6835-6840.2001

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Author Hill, M. K.
Hooker, C. W.
Harrich, D.
Crowe, S. M.
Mak, J.
Title Gag-Pol supplied in trans is efficiently packaged and supports viral function in human immunodeficiency virus type 1
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2001-08-01
Year available 2001
Sub-type Article (original research)
DOI 10.1128/JVI.75.15.6835-6840.2001
Open Access Status File (Publisher version)
Volume 75
Issue 15
Start page 6835
End page 6840
Total pages 6
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
Abstract The intracellular trafficking and subsequent incorporation of Gag-Pol into human immunodeficiency virus type 1 (HIV-1) remains poorly defined. Gag-Pol is encoded by the same mRNA as Gag and is generated by ribosomal frameshifting. The multimerization of Gag and Gag-Pol is an essential step in the formation of infectious viral particles. In this study, we examined whether the interaction between Gag and Gag-Pol is initiated during protein translation in order to facilitate the trafficking and subsequent packaging of Gag-Pol into the virion. A conditional cotransfection system was developed in which virion formation required the coexpression of two HIV-1-based plasmids, one that produces both Gag and Gag-Pol and one that only produces Gag-Pol. The Gag-Pol proteins were either immunotagged with a His epitope or functionally tagged with a mutation (K65R) in reverse transcriptase that is associated with drug resistance. Gag-Pol packaging was assessed to determine whether the Gag-Pol incorporated into the virion was preferentially packaged from the plasmid that expressed both Gag and Gag-Pol or whether it could be packaged from either plasmid. Our data show that translation of Gag and Gag-Pol from the same mRNA is not critical for virion packaging of the Gag-Pol polyprotein or for viral function.
Keyword Virology
Assembly Intermediate Complexes
In-vitro
Reverse-transcriptase
Precursor Protein
Wild-type
Particles
Region
Polyprotein
Virions
Cells
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Wed, 15 Aug 2007, 02:06:10 EST