A virus-based single-enzyme nanoreactor

Comellas-Aragones, Marta, Engelkamp, Hans, Claessen, Victor I., Sommerdijk, Nico A. J. M., Rowan, Alan E., Christianen, Peter C. M., Maan, Jan C., Verduin, Benedictus J. M., Cornelissen, Jeroen J. L. M. and Nolte, Roeland J. M. (2007) A virus-based single-enzyme nanoreactor. Nature Nanotechnology, 2 10: 635-639. doi:10.1038/nnano.2007.299

Author Comellas-Aragones, Marta
Engelkamp, Hans
Claessen, Victor I.
Sommerdijk, Nico A. J. M.
Rowan, Alan E.
Christianen, Peter C. M.
Maan, Jan C.
Verduin, Benedictus J. M.
Cornelissen, Jeroen J. L. M.
Nolte, Roeland J. M.
Title A virus-based single-enzyme nanoreactor
Journal name Nature Nanotechnology   Check publisher's open access policy
ISSN 1748-3387
Publication date 2007-10-01
Year available 2007
Sub-type Article (original research)
DOI 10.1038/nnano.2007.299
Open Access Status Not yet assessed
Volume 2
Issue 10
Start page 635
End page 639
Total pages 5
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract Most enzyme studies are carried out in bulk aqueous solution, at the so-called ensemble level, but more recently studies have appeared in which enzyme activity is measured at the level of a single molecule, revealing previously unseen properties. To this end, enzymes have been chemically or physically anchored to a surface, which is often disadvantageous because it may lead to denaturation. In a natural environment, enzymes are present in a confined reaction space, which inspired us to develop a generic method to carry out single-enzyme experiments in the restricted spatial environment of a virus capsid. We report here the incorporation of individual horseradish peroxidase enzymes in the inner cavity of a virus, and describe single-molecule studies on their enzymatic behaviour. These show that the virus capsid is permeable for substrate and product and that this permeability can be altered by changing pH.
Keyword Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Science & Technology - Other Topics
Materials Science
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 236 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 250 times in Scopus Article | Citations
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