A hydrogel-based enzyme-loaded polymersome reactor

De Hoog, Hans-Peter M., Arends, Isabel W. C. E., Rowan, Alan E., Cornelissen, Jeroen J. L. M. and Nolte, Roeland J. M. (2010) A hydrogel-based enzyme-loaded polymersome reactor. Nanoscale, 2 5: 709-716. doi:10.1039/b9nr00325h

Author De Hoog, Hans-Peter M.
Arends, Isabel W. C. E.
Rowan, Alan E.
Cornelissen, Jeroen J. L. M.
Nolte, Roeland J. M.
Title A hydrogel-based enzyme-loaded polymersome reactor
Journal name Nanoscale   Check publisher's open access policy
ISSN 2040-3364
Publication date 2010-05-01
Year available 2010
Sub-type Article (original research)
DOI 10.1039/b9nr00325h
Open Access Status Not yet assessed
Volume 2
Issue 5
Start page 709
End page 716
Total pages 8
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Subject 2500 Materials Science
Abstract In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs under mild conditions and does not inhibit the activity of the encapsulated enzymes nor does it affect the structure of the polymersomes. The stability of the polymersome hydrogel architecture is demonstrated by the facile recycling of the polymersomes and their use in repeated reaction cycles. A 'continuous-flow polymersome reactor' is constructed in which substrate is added to the top of the reactor and product is collected at the bottom. This set-up allows the use of different enzymes and the processing of multiple substrates, as is demonstrated by the conversion of 2-methoxyphenyl acetate to tetraguaiacol in a reactor loaded with polymersome hydrogels containing the enzymes Candida antarctica lipase B (CALB) and glucose oxidase (GOx).
Keyword Chemistry, Multidisciplinary
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Applied
Science & Technology - Other Topics
Materials Science
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 18 times in Scopus Article | Citations
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