UV-induced hyperphosphorylation of replication protein A depends on DNA replication and expression of ATM protein

Oakley, GG, Loberg, LI, Yao, JQ, Risinger, MA, Yunker, RL, Zernik-Kobak, M, Khanna, KK, Lavin, MF, Carty, MP and Dixon, K (2001) UV-induced hyperphosphorylation of replication protein A depends on DNA replication and expression of ATM protein. Molecular Biology of The Cell, 12 5: 1199-1213.

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Author Oakley, GG
Loberg, LI
Yao, JQ
Risinger, MA
Yunker, RL
Zernik-Kobak, M
Khanna, KK
Lavin, MF
Carty, MP
Dixon, K
Title UV-induced hyperphosphorylation of replication protein A depends on DNA replication and expression of ATM protein
Journal name Molecular Biology of The Cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2001-05-01
Sub-type Critical review of research, literature review, critical commentary
Open Access Status File (Publisher version)
Volume 12
Issue 5
Start page 1199
End page 1213
Total pages 15
Place of publication Bethesda, MD, United States
Publisher American Society for Cell Biology
Language eng
Formatted abstract
Exposure to DNA-damaging agents triggers signal transduction pathways that are thought to play a role in maintenance of genomic stability. A key protein in the cellular processes of nucleotide excision repair, DNA recombination, and DNA double-strand break repair is the single-stranded DNA binding protein, RPA. We showed previously that the p34 subunit of RPA becomes hyperphosphorylated as a delayed response (4-8 h) to UV radiation (10-30 J/m2). Here we show that UV-induced RPA-p34 hyperphosphorylation depends on expression of ATM, the product of the gene mutated in the human genetic disorder ataxia telangiectasia (A-T). UV-induced RPA-p34 hyperphosphorylation was not observed in A-T cells, but this response was restored by ATM expression. Furthermore, purified ATM kinase phosphorylates the p34 subunit of RPA complex in vitro at many of the same sites that are phosphorylated in vivo after UV radiation. Induction of this DNA damage response was also dependent on DNA replication; inhibition of DNA replication by aphidicolin prevented induction of RPA-p34 hyperphosphorylation by UV radiation. We postulate that this pathway is triggered by the accumulation of aberrant DNA replication intermediates, resulting from DNA replication fork blockage by UV photoproducts. Further, we suggest that RPA-p34 is hyperphosphorylated as a participant in the recombinational postreplication repair of these replication products. Successful resolution of these replication intermediates reduces the accumulation of chromosomal aberrations that would otherwise occur as a consequence of UV radiation.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes This document is a journal review.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: UQ Centre for Clinical Research Publications
School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 01:52:23 EST