Class I histone deacetylases sequentially interact with MyoD and pRb during skeletal myogenesis

Puri, P. L., Iezzi, S., Stiegler, P., Chen, T. T., Schiltz, R. L., Muscat, G. E. O., Giordano, A., Kedes, L, Wang, J. Y. J. and Sartorelli, V. (2001) Class I histone deacetylases sequentially interact with MyoD and pRb during skeletal myogenesis. Molecular Cell, 8 4: 885-897. doi:10.1016/S1097-2765(01)00373-2

Author Puri, P. L.
Iezzi, S.
Stiegler, P.
Chen, T. T.
Schiltz, R. L.
Muscat, G. E. O.
Giordano, A.
Kedes, L
Wang, J. Y. J.
Sartorelli, V.
Title Class I histone deacetylases sequentially interact with MyoD and pRb during skeletal myogenesis
Journal name Molecular Cell   Check publisher's open access policy
ISSN 1097-2765
Publication date 2001-01-01
Year available 2001
Sub-type Article (original research)
DOI 10.1016/S1097-2765(01)00373-2
Open Access Status DOI
Volume 8
Issue 4
Start page 885
End page 897
Total pages 13
Place of publication Cambridge, Mass USA
Publisher Cell Press
Language eng
Subject C1
270100 Biochemistry and Cell Biology
780105 Biological sciences
Abstract We describe a functional and biochemical link between the myogenic activator MyoD, the deacetylase HDAC1, and the tumor suppressor pRb. Interaction of MyoD with HDAC1 in undifferentiated myoblasts mediates repression of muscle-specific gene expression. Prodifferentiation cues, mimicked by serum removal, induce both downregulation of HDAC1 protein and pRb hypophosphorylation. Dephosphorylation of pRb promotes the formation of pRb-HDAC1 complex in differentiated myotubes. pRb-HDAC1 association coincides with disassembling of MyoD-HDAC1 complex, transcriptional activation of muscle-restricted genes, and cellular differentiation of skeletal myoblasts. A single point mutation introduced in the HDAC1 binding domain of pRb compromises its ability to disrupt MyoD-HDAC1 interaction and to promote muscle gene expression. These results suggest that reduced expression of HDAC1 accompanied by its redistribution in alternative nuclear protein complexes is critical for terminal differentiation of skeletal muscle cells.
Keyword Biochemistry & Molecular Biology
Cell Biology
Cell-cycle Arrest
Mef2 Transcription Factor
Muscle Gene-expression
Retinoblastoma Protein
Terminal Differentiation
Repress Transcription
Q-Index Code C1
Grant ID R01 CA058320
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 148 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 15 Aug 2007, 01:43:31 EST