Efficiency of delivery of DNA to cells by bovine papillomavirus type-1 L1/L2 pseudovirions

Liu, Y., Frazer, I. H., Liu, W. J., Liu, X. S., McMillan, N. and Zhao, K. N. (2001) Efficiency of delivery of DNA to cells by bovine papillomavirus type-1 L1/L2 pseudovirions. Applied Microbiology And Biotechnology, 56 1-2: 150-156. doi:10.1007/s002530100655


Author Liu, Y.
Frazer, I. H.
Liu, W. J.
Liu, X. S.
McMillan, N.
Zhao, K. N.
Title Efficiency of delivery of DNA to cells by bovine papillomavirus type-1 L1/L2 pseudovirions
Journal name Applied Microbiology And Biotechnology   Check publisher's open access policy
ISSN 0175-7598
Publication date 2001-01-01
Sub-type Article (original research)
DOI 10.1007/s002530100655
Volume 56
Issue 1-2
Start page 150
End page 156
Total pages 7
Editor A. Steinbuechel
Place of publication Germany
Publisher Springer-Verlag
Language eng
Subject C1
270801 Gene Therapy
730101 Infectious diseases
Abstract To investigate the efficiency of encapsidation of plasmid by papillomavirus virus-like particles (PV VLPs), and the infectivity of the resultant PV pseudovirions, Cos-1 cells were transfected with an 8-kb plasmid incorporating a green fluorescent protein (GFP) reporter gene (pGSV), and infected with bovine PV (BPV-1) L1/L2 recombinant vaccinia virus to produce BPV1 pseudovirions. Approximately 1 in 1.5x10(4) of dense (1.35 g/ml) PV pseudovirions and 0.3 in 10(4) Of less-dense (1.29 g/ml) pseudovirions packaged an intact pGSV plasmid. The majority (>75%) of packaged plasmids contained deletions, and the deletions affected all tested genes. After exposure of Cos-1 cells to BPV-1 pseudovirions at an MOI of 40,000:1, 6% of cells expressed GFP giving a calculated efficiency of delivery of the pGSV plasmid, by pseudovirions which had packaged an intact plasmid, of approximately 5%. Plasmid delivery was not effected by purified pGSV plasmid, was blocked by antiserum against BPV-1, and was not blocked by DNase treatment of pseudovirions, confirming that delivery was mediated by DNA within the pseudovirion. We conclude that a major limitation to the use of PV pseudovirions as a gene delivery system is that intact plasmid DNA is not efficiently selected for packaging by VLPs in cell-based pseudovirions production systems.
Keyword Biotechnology & Applied Microbiology
Major Capsid Protein
Virus-like Particles
In-vitro
Plasmid Dna
L1
L2
Generation
Neutralization
Expression
Integrin
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 2 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 01:31:30 EST