The wheat Lr34 multipathogen resistance gene confers resistance to anthracnose and rust in sorghum

Schnippenkoetter, Wendelin, Lo, Clive, Liu, Guoquan, Dibley, Katherine, Chan, Wai Lung, White, Jodie, Milne, Ricky, Zwart, Alexander, Kwong, Eunjung, Keller, Beat, Godwin, Ian, Krattinger, Simon G. and Lagudah, Evans (2017) The wheat Lr34 multipathogen resistance gene confers resistance to anthracnose and rust in sorghum. Plant Biotechnology Journal, 15 11: 1387-1396. doi:10.1111/pbi.12723


Author Schnippenkoetter, Wendelin
Lo, Clive
Liu, Guoquan
Dibley, Katherine
Chan, Wai Lung
White, Jodie
Milne, Ricky
Zwart, Alexander
Kwong, Eunjung
Keller, Beat
Godwin, Ian
Krattinger, Simon G.
Lagudah, Evans
Title The wheat Lr34 multipathogen resistance gene confers resistance to anthracnose and rust in sorghum
Journal name Plant Biotechnology Journal   Check publisher's open access policy
ISSN 1467-7652
1467-7644
Publication date 2017-04-20
Year available 2017
Sub-type Article (original research)
DOI 10.1111/pbi.12723
Open Access Status DOI
Volume 15
Issue 11
Start page 1387
End page 1396
Total pages 10
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Abstract The ability of the wheat Lr34 multipathogen resistance gene (Lr34res) to function across a wide taxonomic boundary was investigated in transgenic Sorghum bicolor. Increased resistance to sorghum rust and anthracnose disease symptoms following infection with the biotrophic pathogen Puccinia purpurea and the hemibiotroph Colletotrichum sublineolum, respectively, occurred in transgenic plants expressing the Lr34res ABC transporter. Transgenic sorghum lines that highly expressed the wheat Lr34res gene exhibited immunity to sorghum rust compared to the low-expressing single copy Lr34res genotype that conferred partial resistance. Pathogen-induced pigmentation mediated by flavonoid phytoalexins was evident on transgenic sorghum leaves following P. purpurea infection within 24-72 h, which paralleled Lr34res gene expression. Elevated expression of flavone synthase II, flavanone 4-reductase and dihydroflavonol reductase genes which control the biosynthesis of flavonoid phytoalexins characterized the highly expressing Lr34res transgenic lines 24-h post-inoculation with P. purpurea. Metabolite analysis of mesocotyls infected with C. sublineolum showed increased levels of 3-deoxyanthocyanidin metabolites were associated with Lr34res expression, concomitant with reduced symptoms of anthracnose.
Formatted abstract
The ability of the wheat Lr34 multipathogen resistance gene (Lr34res) to function across a wide taxonomic boundary was investigated in transgenic Sorghum bicolor. Increased resistance to sorghum rust and anthracnose disease symptoms following infection with the biotrophic pathogen Puccinia purpurea and the hemibiotroph Colletotrichum sublineolum, respectively, occurred in transgenic plants expressing the Lr34res ABC transporter. Transgenic sorghum lines that highly expressed the wheat Lr34res gene exhibited immunity to sorghum rust compared to the low-expressing single copy Lr34res genotype that conferred partial resistance. Pathogen-induced pigmentation mediated by flavonoid phytoalexins was evident on transgenic sorghum leaves following P. purpurea infection within 24-72 h, which paralleled Lr34res gene expression. Elevated expression of flavone synthase II, flavanone 4-reductase and dihydroflavonol reductase genes which control the biosynthesis of flavonoid phytoalexins characterized the highly expressing Lr34res transgenic lines 24-h post-inoculation with P. purpurea. Metabolite analysis of mesocotyls infected with C. sublineolum showed increased levels of 3-deoxyanthocyanidin metabolites were associated with Lr34res expression, concomitant with reduced symptoms of anthracnose.
Keyword Anthracnose
Flavonoid phytoalexin
Lr34
Multiple disease resistance
Rust
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID OPP1060218
17 123 315
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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