Properties and function of KCNQ1 K+ channels isolated from the rectal gland of Squalus acanthias

Kerst, G., Beschorner, U., Unsold, B., von Hahn, T., Schreiber, R., Greger, R., Gerlach, U., Lang, H. J., Kunzelmann, K. and Bleich, M. (2001) Properties and function of KCNQ1 K+ channels isolated from the rectal gland of Squalus acanthias. Pflugers Archiv european Journal of Physiology, 443 1: 146-154. doi:10.1007/s004240100632

Author Kerst, G.
Beschorner, U.
Unsold, B.
von Hahn, T.
Schreiber, R.
Greger, R.
Gerlach, U.
Lang, H. J.
Kunzelmann, K.
Bleich, M.
Title Properties and function of KCNQ1 K+ channels isolated from the rectal gland of Squalus acanthias
Journal name Pflugers Archiv european Journal of Physiology   Check publisher's open access policy
ISSN 0031-6768
Publication date 2001-01-01
Sub-type Article (original research)
DOI 10.1007/s004240100632
Volume 443
Issue 1
Start page 146
End page 154
Total pages 9
Place of publication Germany
Publisher Springer-Verlag
Language eng
Subject C1
270104 Membrane Biology
730110 Respiratory system and diseases (incl. asthma)
1103 Clinical Sciences
Abstract KCNQ1 (K(V)LQT1) K+ channels play an important role during electrolyte secretion in airways and colon. KCNQ1 was cloned recently from NaCl-secreting shark rectal glands. Here we study. the properties and regulation of the cloned sK(V)LQT1 expressed in Xenopus oocytes and Chinese hamster ovary (CHO) cells and compare the results with those obtained from in vitro perfused rectal gland tubules (RGT). The expression of sKCNQ1 induced voltage-dependent, delayed activated K+ currents, which were augmented by an increase in intracellular cAMP and Ca2+. The chromanol derivatives 293B and 526B potently inhibited sKCNQ1 expressed in oocytes and CHO cells, but had little effect on RGT electrolyte transport. Short-circuit currents in RGT were activated by alkalinization and were decreased by acidification. In CHO cells an alkaline pH activated and an acidic pH inhibited 293B-sensitive KCNQ1 currents. Noise analysis of the cell-attached basolateral membrane of RGT indicated the presence of low-conductance (<3 pS) K+ channels, in parallel with other K+ channels. sKCNQ1 generated similar small-conductance K+ channels upon expression in CHO cells and Xenopus oocytes. The results suggest the presence of low-conductance KCNQ1 K+ channels in RGT, which are probably regulated by changes in intracellular cAMP, Ca2+ and pH.
Keyword Physiology
Cl- Secretion
Epithelial Transport
Squalus Acanthias
Mediated Cl Secretion
Potassium Channel
Basolateral Membrane
Nacl Secretion
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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Created: Wed, 15 Aug 2007, 01:11:15 EST