A fluorometric microassay for histamine release from human gingival mast cells

Zhao, Z. Z., Sugerman, P. B., Walsh, L. J. and Savage, N. W. (2001) A fluorometric microassay for histamine release from human gingival mast cells. Journal of Periodontal Research, 36 4: 233-236. doi:10.1034/j.1600-0765.2001.036004233.x


Author Zhao, Z. Z.
Sugerman, P. B.
Walsh, L. J.
Savage, N. W.
Title A fluorometric microassay for histamine release from human gingival mast cells
Journal name Journal of Periodontal Research   Check publisher's open access policy
ISSN 0022-3484
Publication date 2001-01-01
Year available 2001
Sub-type Article (original research)
DOI 10.1034/j.1600-0765.2001.036004233.x
Open Access Status
Volume 36
Issue 4
Start page 233
End page 236
Total pages 4
Editor R. C. Page
Place of publication Denmark
Publisher Munksgaard
Language eng
Subject C1
320899 Dentistry not elsewhere classified
730112 Oro-dental and disorders
Abstract Mast cells are important effector cells of the immune system. We describe a rapid and inexpensive microassay to determine histamine release from human gingival mast cells. The assay is based on the coupling of histamine with o-phthalaldehyde (OPT) at a highly alkaline pH to form a fluorescent product. Using this assay with a sample volume of 10 mul/well in a 384 black well microplate, the histamine detection limit was 0.031 mug/ml. The human mast cell line (HMC-1) and fresh mast cells isolated from human gingival tissue (n = 10) were stimulated with substance P, anti-IgE or calcium ionophore A23187, Calcium ionophore significantly increased histamine release from HMC-1 cells and gingival mast cells (p < 0.05). This microassay will facilitate the study of mast cell histamine release in diseased oral mucosa.
Keyword Dentistry, Oral Surgery & Medicine
Mast Cells
Histamine
Microassay
Lichen-planus
Substance-p
Receptor
Line
Skin
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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Created: Wed, 15 Aug 2007, 00:52:51 EST