Is CuII coordinated to patellamides inside Prochloron cells?

Comba, Peter, Eisenschmidt, Annika, Gahan, Lawrence R., Herten, Dirk-Peter , Nette, Geoffrey, Schenk, Gerhard and Seefeld, Martin (2017) Is CuII coordinated to patellamides inside Prochloron cells?. Chemistry - A European Journal, 23 50: 12264-12274. doi:10.1002/chem.201700895


Author Comba, Peter
Eisenschmidt, Annika
Gahan, Lawrence R.
Herten, Dirk-Peter
Nette, Geoffrey
Schenk, Gerhard
Seefeld, Martin
Title Is CuII coordinated to patellamides inside Prochloron cells?
Formatted title
Is CuII coordinated to patellamides inside Prochloron cells?
Journal name Chemistry - A European Journal   Check publisher's open access policy
ISSN 1521-3765
0947-6539
Publication date 2017-04-26
Year available 2017
Sub-type Article (original research)
DOI 10.1002/chem.201700895
Open Access Status Not yet assessed
Volume 23
Issue 50
Start page 12264
End page 12274
Total pages 12
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Language eng
Subject 1600 Chemistry
Abstract Dinuclear Cu-II-patellamide complexes (patellamides are naturally occurring cyclic pseudo-octapeptides) are known to be efficient catalysts for hydrolysis reactions of biological importance, for example, those of phosphatase, carbonic anhydrase, and glycosidase. However, the biological role of patellamides is still unknown. Patellamides were originally extracted from the sea squirt Lissoclinum patella, but are now known to be ribosomally expressed by the blue-green algae Prochloron that live in symbiosis with L. patella. In a further step to unravel the metabolic significance of the patellamide complexes, the question as to whether these are also formed inside Prochloron cells is addressed. In this study, a biocompatible patellamide-fluorescent dye conjugate has been introduced into living Prochloron cells and, by means of flow cytometry and confocal microscopy, it is shown that Cu-II ions are coordinated to patellamides in vivo.
Formatted abstract
Dinuclear CuII–patellamide complexes (patellamides are naturally occurring cyclic pseudo-octapeptides) are known to be efficient catalysts for hydrolysis reactions of biological importance, for example, those of phosphatase, carbonic anhydrase, and glycosidase. However, the biological role of patellamides is still unknown. Patellamides were originally extracted from the sea squirt Lissoclinum patella, but are now known to be ribosomally expressed by the blue-green algae Prochloron that live in symbiosis with L. patella. In a further step to unravel the metabolic significance of the patellamide complexes, the question as to whether these are also formed inside Prochloron cells is addressed. In this study, a biocompatible patellamide–fluorescent dye conjugate has been introduced into living Prochloron cells and, by means of flow cytometry and confocal microscopy, it is shown that CuII ions are coordinated to patellamides in vivo.
Keyword Copper
Cyanobacteria
Fluorescence
Marine organisms
Patellamides
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID bwHPC-C5
FT120100694
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 05 May 2017, 11:10:29 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences