Plant transformation: Problems and strategies for practical application

Birch, RG (1997) Plant transformation: Problems and strategies for practical application. Annual Review of Plant Physiology And Plant Molecular Biology, 48 297-326. doi:10.1146/annurev.arplant.48.1.297


Author Birch, RG
Title Plant transformation: Problems and strategies for practical application
Journal name Annual Review of Plant Physiology And Plant Molecular Biology   Check publisher's open access policy
ISSN 1040-2519
Publication date 1997-01-01
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1146/annurev.arplant.48.1.297
Volume 48
Start page 297
End page 326
Total pages 30
Language eng
Abstract Plant transformation is now a core research tool in plant biology and a practical tool for cultivar improvement. There are verified methods for stable introduction of novel genes into the nuclear genomes of over 120 diverse plant species. This review examines the criteria to verify plant transformation; the biological and practical requirements for transformation systems; the integration of tissue culture, gene transfer, selection, and transgene expression strategies to achieve transformation in recalcitrant species; and other constraints to plant transformation including regulatory environment, public perceptions, intellectual property, and economics. Because the costs of screening populations showing diverse genetic changes can far exceed the costs of transformation, it is important to distinguish absolute and useful transformation efficiencies. The major technical challenge facing plant transformation biology is the development of methods and constructs to produce a high proportion of plants showing predictable transgene expression without collateral genetic damage. This will require answers to a series of biological and technical questions, some of which are defined.
Keyword Biochemistry & Molecular Biology
Plant Sciences
Plant Improvement
Gene Transfer
Transgenic Plants
Transgene Expression
Genetic Engineering
Transgenic Tobacco Plants
Mediated Gene-transfer
T-dna
Agrobacterium-tumefaciens
Microprojectile Bombardment
Foreign Genes
Intellectual Property
Particle Bombardment
Maize Plants
Stable Transformation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: School of Biological Sciences Publications
 
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Created: Tue, 14 Aug 2007, 02:50:42 EST