Odorant-induced currents in intact patches from rat olfactory receptor neurons: Theory and experiment

Chiu, P, Lynch, JW and Barry, PH (1997) Odorant-induced currents in intact patches from rat olfactory receptor neurons: Theory and experiment. Biophysical Journal, 72 3: 1442-1457.

Author Chiu, P
Lynch, JW
Barry, PH
Title Odorant-induced currents in intact patches from rat olfactory receptor neurons: Theory and experiment
Journal name Biophysical Journal   Check publisher's open access policy
ISSN 0006-3495
Publication date 1997-01-01
Year available 1997
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 72
Issue 3
Start page 1442
End page 1457
Total pages 16
Place of publication BETHESDA
Publisher BIOPHYSICAL SOCIETY
Language eng
Abstract Odorant-induced currents in mammalian olfactory receptor neurons have proved difficult to obtain reliably using conventional whole-cell recording. By using a mathematical model of the electrical circuit of the patch and rest-of-cell, we demonstrate how cell-attached patch measurements can be used to quantitatively analyze responses to odorants or a high (100 mM) K+ solution. High K+ induced an immediate current flux from cell to pipette, which was modeled as a depolarization of similar to 52 mV, close to that expected from the Nernst equation (56 mV), and no change in the patch conductance. By contrast, a cocktail of cAMP-stimulating odorants induced a current flux from pipette into cell following a significant (4-10 s) delay. This was modeled as an average patch conductance increase of 36 pS and a depolarization of 13 mV, Odorant-induced single channels had a conductance of 16 pS. In cells bathed with no Mg2+ and 0.25 mM Ca2+, odorants induced a current flow from cell to pipette, which was modeled as a patch conductance increase of similar to 115 pS and depolarization of similar to 32 mV, All these results are consistent with cAMP-gated cation channels dominating the odorant response, This approach, which provides useful estimates of odorant-induced voltage and conductance changes, is applicable to similar measurements in any small cells.
Keyword Biophysics
Nucleotide-gated Channels
Cyclic-nucleotide
Action-potentials
Software Package
Transduction
Cells
Calcium
Clamp
Conductance
Activation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 02:41:05 EST