Sulfated galactans from Australian specimens of the red alga Phacelocarpus peperocarpos (Gigartinales, Rhodophyta)

Liao, ML, Chiovitti, A, Munro, SLA, Craik, DJ, Kraft, GT and Bacic, A (1996) Sulfated galactans from Australian specimens of the red alga Phacelocarpus peperocarpos (Gigartinales, Rhodophyta). Carbohydrate Research, 296 237-247. doi:10.1016/S0008-6215(96)00232-7

Author Liao, ML
Chiovitti, A
Munro, SLA
Craik, DJ
Kraft, GT
Bacic, A
Title Sulfated galactans from Australian specimens of the red alga Phacelocarpus peperocarpos (Gigartinales, Rhodophyta)
Journal name Carbohydrate Research   Check publisher's open access policy
ISSN 0008-6215
Publication date 1996-01-01
Year available 1996
Sub-type Article (original research)
DOI 10.1016/S0008-6215(96)00232-7
Open Access Status
Volume 296
Start page 237
End page 247
Total pages 11
Place of publication OXFORD
Language eng
Subject 1602 Analytical Chemistry
1303 Biochemistry
1605 Organic Chemistry
Abstract Polysaccharides from the red alga Phacelocarpos peperocarpos were extracted with hot water, clarified, and precipitated with 2-propanol. The native preparation was highly sulfated (36.2% w/w). Alkali modification decreased the sulfate content by 2.0% w/w. The alkali-modified polysaccharide is composed mostly of galactose (Gal, 51 mol%) and 3,6-anhydrogalactose (AnGal, 41 mol%), with minor amounts of a mono-O-methylgalactose (MeGal, 1 mol%), xylose (Xyl, 6 mol%), and glucose (Glc, 1 mol%). The FTIR spectrum of the alkali-modified polysaccharide resembled K-carrageenan with absorption at 930 cm(-1) (indicative of AnGal) and 850 cm(-1) (Gal ii-sulfate). However, an additional, major band of absorption occurred at 820 cm(-1) indicating the presence of equatorial sulfate ester substitution at O-6 of Gal residues, A combination of linkage and C-13 NMR spectroscopic analyses showed that the polysaccharide was composed predominantly of a novel repeating-unit, O-beta-D-galactopyranosyl 4,6-disulfate)-(1 --> 4)-3,6-anhydro-alpha-D-galactopyranose. Minor structural variations also occurred, including alternative patterns of sulfation and the presence of terminal Xylp, The location of the terminal Xylp residues was not certain but evidence supported their attachment at O-3 of some 4-linked Galp residues. The cell-wall galactans remain unchanged during the life cycle of the alga. (C) 1996 Elsevier Science Ltd.
Keyword Biochemistry & Molecular Biology
Chemistry, Applied
Chemistry, Organic
Carrageenan 4',6'-disulfated Kappa-, And Omega-
Galactans, Sulfated
Structure Determination
Polysaccharides, Algal
J Ag Rhodophyta
C-13-nmr Spectroscopy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
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Created: Tue, 14 Aug 2007, 02:36:46 EST