Functional Organization of the GluR1 Glutamate Receptor Promoter

Borges, K and Dingledine, R (2001) Functional Organization of the GluR1 Glutamate Receptor Promoter. Journal of Biological Chemistry, 276 28: 25929-25938. doi:10.1074/jbc.M009105200

Author Borges, K
Dingledine, R
Title Functional Organization of the GluR1 Glutamate Receptor Promoter
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
Publication date 2001-07-01
Year available 2001
Sub-type Article (original research)
DOI 10.1074/jbc.M009105200
Open Access Status
Volume 276
Issue 28
Start page 25929
End page 25938
Total pages 10
Place of publication BETHESDA
Language eng
Abstract The GluR1 glutamate receptor subunit is expressed in most brain areas and plays a major role in excitatory synaptic transmission. We cloned and sequenced 5 kilobase pairs of the rat GluR1 promoter and identified multiple transcriptional start sites between -295 and -202 (relative to the first ATG). Similar to other glutamate receptor subunit promoters, the GluR1 promoter lacks TATA and CAAT elements in that region but binds Spl proteins at two sites. Promoter activity of GluR1 fragments cloned into pGL3 was assessed by immunocytochemistry and by measuring luciferase activity after transfection into primary cultures of rat cortical neurons and glia. GluR1 promoter activity was stronger in neurons, with neuronal specificity appearing to reside mainly within the neuronal expression-enhancing regions, -1395 to -743 and -253 to -48. The latter region contains 4 sites that bound recombinant cAMP-response element-binding proteins and a glial silencing region between -253 and -202. In both neurons and dia, promoter activity was increased by a 64-base pair GA repeat upstream of the initiation sites and reduced by a 57-base pair region that contained an N box. In contrast to the GluR2 promoter the regulatory regions are mainly located outside of the GluR1 initiation region.
Keyword Biochemistry & Molecular Biology
Biochemistry & Molecular Biology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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