The assessment of oral cytology utilizing ploidy analysis and virtual microscopy for the early detection of epithelial dysplasia and neoplasia in oral mucosal lesions

McCullough, M. J. and Farah, C. S. (2006). The assessment of oral cytology utilizing ploidy analysis and virtual microscopy for the early detection of epithelial dysplasia and neoplasia in oral mucosal lesions. In: Journal of Oral Pathology & Medicine: International Association of Oral Pathologists. The 13th International Congress on Oral Pathology and Medicine Abstracts. The 13th International Congress on Oral Pathology and Medicine, Brisbane, Australia, (427-428). 21 - 25 June, 2006. doi:10.1111/j.1600-0714.2006.00463.x


Author McCullough, M. J.
Farah, C. S.
Title of paper The assessment of oral cytology utilizing ploidy analysis and virtual microscopy for the early detection of epithelial dysplasia and neoplasia in oral mucosal lesions
Conference name The 13th International Congress on Oral Pathology and Medicine
Conference location Brisbane, Australia
Conference dates 21 - 25 June, 2006
Proceedings title Journal of Oral Pathology & Medicine: International Association of Oral Pathologists. The 13th International Congress on Oral Pathology and Medicine Abstracts   Check publisher's open access policy
Journal name Journal of Oral Pathology   Check publisher's open access policy
Place of Publication Copenhagen
Publisher Munksgaard
Publication Year 2006
DOI 10.1111/j.1600-0714.2006.00463.x
ISSN 0904-2512
Volume 35
Issue 7
Start page 427
End page 428
Total pages 2
Language eng
Abstract/Summary Oral squamous cell carcinoma (OSCC) is associated with high morbidity and mortality which is due, at least in part, to late detection. Precancerous and cancerous oral lesions may mimic any number of benign oral lesions, and as such may be left without investigation and treatment until they are well advanced. Over the past several years there has been renewed interest in oral cytology as an adjuvant clinical tool in the investigation of oral mucosal lesions. The purpose of the present study was to compare the usefulness of ploidy analysis after Feulgen stained cytological thin-prep specimens with traditional incisional biopsy and routine histopathological examination for the assessment of the pre-malignant potential of oral mucosal lesions. An analysis of the cytological specimens was undertaken with virtual microscopy which allowed for rapid and thorough analysis of the complete cytological specimen. 100 healthy individuals between 30 and 70 years of age, who were non-smokers, non-drinkers and not taking any medication, had cytological specimens collected from both the buccal mucosa and lateral margin of tongue to establish normal cytology parameters within a control population. Patients with a presumptive clinical diagnosis of lichen planus, leukoplakia or OSCC had lesional cytological samples taken prior to their diagnostic biopsy. Standardised thin preparations were prepared and each specimen stained by both Feuglen and Papanicolau methods. High speed scanning of the complete slide at 40X magnification was undertaken using the Aperio Scanscope TM and the green channel of the resultant image was analysed after threshold segmentation to isolate only nuclei and the integrated optical density of each nucleus taken as a gross measure of the DNA content (ploidy). Preliminary results reveal that ploidy assessment of oral cytology holds great promise as an adjunctive prognostic factor in the analysis of the malignant potential of oral mucosal lesions.
Subjects CX
Keyword Dentistry, Oral Surgery & Medicine
Pathology
Q-Index Code CX

 
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Created: Tue, 14 Aug 2007, 02:21:12 EST