Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment

Jing, DB, Li, PJ, Stagnitti, F, Xiong, XZ and Li, L (2006) Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment. Ecotoxicology And Environmental Safety, 64 2: 244-250. doi:10.1016/j.econenv.2005.01.002

Author Jing, DB
Li, PJ
Stagnitti, F
Xiong, XZ
Li, L
Title Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment
Journal name Ecotoxicology And Environmental Safety   Check publisher's open access policy
ISSN 0147-6513
Publication date 2006-01-01
Year available 2006
Sub-type Article (original research)
DOI 10.1016/j.econenv.2005.01.002
Open Access Status Not yet assessed
Volume 64
Issue 2
Start page 244
End page 250
Total pages 7
Place of publication San Diego
Publisher Academic Press Inc Elsevier Science
Language eng
Abstract The Ordos Plateau in China is covered with up to 300,000 ha of peashrub (Caragana) which is the dominant natural vegetation and ideal for fodder production. To exploit peashrub fodder, it is crucially important to optimize the culture conditions, especially culture substrate to produce pectinase complex. In this study, a new prescription process was developed. The process, based on a uniform experimental design, first optimizes the solid substrate and second, after incubation, applies two different temperature treatments (30 degrees C for the first 30 h and 23 degrees C for the second 42 h) in the fermentation process. A multivariate regression analysis is applied to a number of independent variables (water, wheat bran, rice dextrose, ammonium sulfate, and Tween 80) to develop a predictive model of pectinase activity. A second-degree polynomial model is developed which accounts for an excellent proportion of the explained variation (R-2 = 97.7%). Using unconstrained mathematical programming, an optimized substrate prescription for pectinase production is subsequently developed. The mathematical analysis revealed that the optimal formula for pectinase production from Aspergillus niger by solid fermentation under the conditions of natural aeration, natural substrate pH (about 6.5), and environmental humidity of 60% is rice dextrose 8%, wheat bran 24%, ammonium sulfate ((NH4)(2)SO4) 6%, and water 61%. Tween 80 was found to have a negative effect on the production of pectinase in solid substrate. With this substrate prescription, pectinase produced by solid fermentation of A. niger reached 36.3IU/(gDM). Goats fed on the pectinase complex obtain an incremental increase of 0.47 kg day(-1) during the initial 25 days of feeding, which is a very promising new feeding prospect for the local peashrub. It is concluded that the new formula may be very useful for the sustainable development of and and semiarid pastures such as those of the Ordos Plateau. (c) 2005 Elsevier Inc. All rights reserved.
Keyword Environmental Sciences
Aspergillus Niger
Solid Fermentation
Prescription Experiment
Multiple Regression
Cellulase Production
State Fermentation
Q-Index Code C1
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Civil Engineering Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 21 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 02:18:21 EST