Pharmacological screening technologies for venom peptide discovery

Prashanth, Jutty Rajan, Hasaballah, Nojod and Vetter, Irina (2017) Pharmacological screening technologies for venom peptide discovery. Neuropharmacology, 127 4-19. doi:10.1016/j.neuropharm.2017.03.038

Author Prashanth, Jutty Rajan
Hasaballah, Nojod
Vetter, Irina
Title Pharmacological screening technologies for venom peptide discovery
Journal name Neuropharmacology   Check publisher's open access policy
ISSN 1873-7064
Publication date 2017-04-01
Year available 2017
Sub-type Article (original research)
DOI 10.1016/j.neuropharm.2017.03.038
Open Access Status Not yet assessed
Volume 127
Start page 4
End page 19
Total pages 16
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Subject 3004 Pharmacology
2804 Cellular and Molecular Neuroscience
Abstract Venomous animals occupy one of the most successful evolutionary niches and occur on nearly every continent. They deliver venoms via biting and stinging apparatuses with the aim to rapidly incapacitate prey and deter predators. This has led to the evolution of venom components that act at a number of biological targets - including ion channels, G-protein coupled receptors, transporters and enzymes - with exquisite selectivity and potency, making venom-derived components attractive pharmacological tool compounds and drug leads. In recent years, plate-based pharmacological screening approaches have been introduced to accelerate venom-derived drug discovery. A range of assays are amenable to this purpose, including high-throughput electrophysiology, fluorescence-based functional and binding assays. However, despite these technological advances, the traditional activity-guided fractionation approach is time-consuming and resource-intensive. The combination of screening techniques suitable for miniaturization with sequence-based discovery approaches - supported by advanced proteomics, mass spectrometry, chromatography as well as synthesis and expression techniques - promises to further improve venom peptide discovery.Here, we discuss practical aspects of establishing a pipeline for venom peptide drug discovery with a particular emphasis on pharmacology and pharmacological screening approaches.
Keyword Activity-guided fractionation
Venom drug discovery
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID FT130101215
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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Institute for Molecular Bioscience - Publications
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