Effect of hypothalamo-pituitary disconnection on the development of anterior pituitary cells in late gestation ovine fetuses

Schwartz, J., Farrand, K., McMillen, I. C., Young, I. R., Houghton, D. and Szarek, E. (2006) Effect of hypothalamo-pituitary disconnection on the development of anterior pituitary cells in late gestation ovine fetuses. Faseb Journal, 20 5: A1283-A1284.


Author Schwartz, J.
Farrand, K.
McMillen, I. C.
Young, I. R.
Houghton, D.
Szarek, E.
Title Effect of hypothalamo-pituitary disconnection on the development of anterior pituitary cells in late gestation ovine fetuses
Journal name Faseb Journal   Check publisher's open access policy
ISSN 0892-6638
Publication date 2006-01-01
Sub-type Published abstract
Volume 20
Issue 5
Start page A1283
End page A1284
Total pages 2
Place of publication Bethesda, MD
Publisher Federation of American Societies for Experimental Biology
Language eng
Abstract The specific role of the hypothalamus in regulating the developmental profile of anterior pituitary (AP) cells remains largely unknown. The present study evaluated hypothalamic contributions to AP cell development, utilizing the technique of hypothalamo-pituitary disconnection (HPD). HPD of fetal sheep or sham surgery was performed at 110 days gestation (d) (n=6 each group; term ~ 147d). Fetuses were removed and pituitaries collected at 110d (no surgery; n=6) or 141d (sham and HPD groups). The impact of HPD on AP cell development was assessed by single-labeled immunofluorescence for five hormones to identify proportions of AP cells expressing each hormone. HPD was associated with a 70% increase (P<0.05) in relative thyrotroph numbers. Following HPD the gestational age-related increase in gonadotroph numbers did not occur (P<0.05). In contrast, the gestational age related increase in lactotrophs was unaffected by HPD. The proportions of somatotrophs and corticotrophs were unaffected by either gestational age or HPD. These data (Figure 1) indicate that AP cells can develop in the absence of an intact hypothalamo-pituitary connection after 110 days gestation but that hypothalamic factors are essential for maintenance of appropriate proportions of thyrotrophs and gonadotrophs during late gestation in the ovine fetus.
Keyword Biochemistry & Molecular Biology
Biology
Cell Biology
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Published abstract
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 02:05:42 EST