Within-host evolution of Burkholderia pseudomallei during chronic infection of seven Australasian cystic fibrosis patients

Viberg, Linda T., Sarovich, Derek S., Kidd, Timothy J., Geake, James B., Bell, Scott C., Currie, Bart J. and Price, Erin P. (2017) Within-host evolution of Burkholderia pseudomallei during chronic infection of seven Australasian cystic fibrosis patients. mBio, 8 2: . doi:10.1128/mBio.00356-17


Author Viberg, Linda T.
Sarovich, Derek S.
Kidd, Timothy J.
Geake, James B.
Bell, Scott C.
Currie, Bart J.
Price, Erin P.
Title Within-host evolution of Burkholderia pseudomallei during chronic infection of seven Australasian cystic fibrosis patients
Formatted title
Within-host evolution of Burkholderia pseudomallei during chronic infection of seven Australasian cystic fibrosis patients
Journal name mBio   Check publisher's open access policy
ISSN 2150-7511
Publication date 2017-04-11
Sub-type Article (original research)
DOI 10.1128/mBio.00356-17
Open Access Status DOI
Volume 8
Issue 2
Total pages 16
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2018
Language eng
Formatted abstract
Cystic fibrosis (CF) is a genetic disorder characterized by progressive lung function decline. CF patients are at an increased risk of respiratory infections, including those by the environmental bacterium Burkholderia pseudomallei, the causative agent of melioidosis. Here, we compared the genomes of B. pseudomallei isolates collected between ~4 and 55 months apart from seven chronically infected CF patients. Overall, the B. pseudomallei strains showed evolutionary patterns similar to those of other chronic infections, including emergence of antibiotic resistance, genome reduction, and deleterious mutations in genes involved in virulence, metabolism, environmental survival, and cell wall components. We documented the first reported B. pseudomallei hypermutators, which were likely caused by defective MutS. Further, our study identified both known and novel molecular mechanisms conferring resistance to three of the five clinically important antibiotics for melioidosis treatment. Our report highlights the exquisite adaptability of microorganisms to long-term persistence in their environment and the ongoing challenges of antibiotic treatment in eradicating pathogens in the CF lung. Convergent evolution with other CF pathogens hints at a degree of predictability in bacterial evolution in the CF lung and potential targeted eradication of chronic CF infections in the future.
Keyword Burkholderia pseudomallei
Antibiotic resistance
Cystic fibrosis
Melioidosis
Parallelism
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 21 Apr 2017, 11:59:22 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences