Effect of sugarcane fiber digestibility, conservation method and concentrate level on the ruminal ecosystem of beef cattle

de Souza, Johnny Maciel, de Sousa, Dannylo Oliveira, de Mesquita, Bruno Souza, Mesquita, Ligia Garcia and Prada Silva, Luis Felipe (2017) Effect of sugarcane fiber digestibility, conservation method and concentrate level on the ruminal ecosystem of beef cattle. AMB Express, 7 1: 55. doi:10.1186/s13568-017-0356-7


Author de Souza, Johnny Maciel
de Sousa, Dannylo Oliveira
de Mesquita, Bruno Souza
Mesquita, Ligia Garcia
Prada Silva, Luis Felipe
Title Effect of sugarcane fiber digestibility, conservation method and concentrate level on the ruminal ecosystem of beef cattle
Journal name AMB Express   Check publisher's open access policy
ISSN 2191-0855
Publication date 2017-03-06
Year available 2017
Sub-type Article (original research)
DOI 10.1186/s13568-017-0356-7
Open Access Status DOI
Volume 7
Issue 1
Start page 55
Total pages 9
Place of publication Heidelberg, Germany
Publisher SpringerOpen
Language eng
Subject 1304 Biophysics
2402 Applied Microbiology and Biotechnology
Abstract The aim of this study was to investigate the effects of sugarcane neutral detergent fiber digestibility (NDFD), conservation method, and concentrate level on the ruminal microbial population of steers. Eight ruminal-cannulated Nellore steers were distributed in two contemporary 4 × 4 Latin Square design with a 2 × 2 factorial arrangement of treatments. Experiment 1: diets were formulated with 60% of concentrate level, and two sugarcane genotypes (high or low NDFD) either freshly cut or as silage. Experiment 2: diets were formulated with two levels of concentrate (60 or 80%), and two sugarcane genotypes (high or low NDFD) offered as freshly cut. Each experimental period lasted for 14 d, with the last 4 d used for ruminal fluid collection. Three cellulolytic bacteria (Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens), two amylolytic (Streptococcus bovis, Ruminobacter amylophilus), and a lactate fermenting microorganism (Megasphaera elsdenii) were quantified by qPCR. Experiment 1: diets with fresh sugarcane increased the population of S. bovis, and M. elsdenii. Sugarcane with high NDFD increased F. succinogenes population only when sugarcane was offered as freshly cut. Experiment 2: increasing concentrate in the diet decreased S. bovis population, and increased R. amylophilus. Sugarcane with high NDFD increased the population of cellulolytic bacteria only at the 60% concentrate diet. Providing sugarcane with high NDFD favored the growth of fibrolytic bacteria, and this effect were dependent on the conservation method and on diet concentrate level. In addition, sucrose appears to have great effect on the composition of ruminal microflora, especially S. bovis.
Formatted abstract
The aim of this study was to investigate the effects of sugarcane neutral detergent fiber digestibility (NDFD), conservation method, and concentrate level on the ruminal microbial population of steers. Eight ruminal-cannulated Nellore steers were distributed in two contemporary 4 × 4 Latin Square design with a 2 × 2 factorial arrangement of treatments. Experiment 1: diets were formulated with 60% of concentrate level, and two sugarcane genotypes (high or low NDFD) either freshly cut or as silage. Experiment 2: diets were formulated with two levels of concentrate (60 or 80%), and two sugarcane genotypes (high or low NDFD) offered as freshly cut. Each experimental period lasted for 14 d, with the last 4 d used for ruminal fluid collection. Three cellulolytic bacteria (Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens), two amylolytic (Streptococcus bovis, Ruminobacter amylophilus), and a lactate fermenting microorganism (Megasphaera elsdenii) were quantified by qPCR. Experiment 1: diets with fresh sugarcane increased the population of S. bovis, and M. elsdenii. Sugarcane with high NDFD increased F. succinogenes population only when sugarcane was offered as freshly cut. Experiment 2: increasing concentrate in the diet decreased S. bovis population, and increased R. amylophilus. Sugarcane with high NDFD increased the population of cellulolytic bacteria only at the 60% concentrate diet. Providing sugarcane with high NDFD favored the growth of fibrolytic bacteria, and this effect were dependent on the conservation method and on diet concentrate level. In addition, sucrose appears to have great effect on the composition of ruminal microflora, especially S. bovis.
Keyword Beef cattle
qPCR
Ruminal fermentation
Ruminal microorganisms
Sugarcane
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 2009/00976-2
2013/22811-0
Institutional Status Non-UQ
Additional Notes Article number 55

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Alliance for Agriculture and Food Innovation
 
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Created: Fri, 21 Apr 2017, 11:46:05 EST by Luis Prada E Silva on behalf of Learning and Research Services (UQ Library)