Analysis of positive control STR experiments reveals that results obtained for FGA, D3S1358, and D13S317 condition the success rate of the analysis of routine reference samples

Murigneux, Valentine, Dufour, Anne-Beatrice, Lobry, Jean R. and Pene, Laurent (2014) Analysis of positive control STR experiments reveals that results obtained for FGA, D3S1358, and D13S317 condition the success rate of the analysis of routine reference samples. Journal of Forensic Sciences, 59 4: 1074-1079. doi:10.1111/1556-4029.12360


Author Murigneux, Valentine
Dufour, Anne-Beatrice
Lobry, Jean R.
Pene, Laurent
Title Analysis of positive control STR experiments reveals that results obtained for FGA, D3S1358, and D13S317 condition the success rate of the analysis of routine reference samples
Journal name Journal of Forensic Sciences   Check publisher's open access policy
ISSN 1556-4029
0022-1198
Publication date 2014-07-01
Sub-type Article (original research)
DOI 10.1111/1556-4029.12360
Open Access Status Not yet assessed
Volume 59
Issue 4
Start page 1074
End page 1079
Total pages 6
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Language eng
Abstract About 120,000 reference samples are analyzed each year in the Forensic Laboratory of Lyon. A total of 1640 positive control experiments used to validate and optimize the analytical method in the routine process were submitted to a multivariate exploratory data analysis approach with the aim of better understanding the underlying sources of variability. The peak heights of the 16 genetic markers targeted by the AmpFℓSTR® Identifiler® STR kit were used as variables of interest. Six different 3130xl genetic analyzers located in the same controlled environment were involved. Two major sources of variability were found: (i) the DNA load of the sample modulates all peak heights in a similar way so that the 16 markers are highly correlated, (ii) the genetic analyzer used with a locus-specific response for peak height and a better sensitivity for the most recently acquired. Three markers (FGA, D3S1358, and D13S317) were found to be of special interest to predict the success rate observed in the routine process.
Keyword Forensic science
FTA card
Identifiler
Multivariate analysis
Peak height
Positive control experiment
Short tandem repeat
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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