Substrate topography and size determine the fate of human embryonic stem cells to neuronal or glial lineage

Ankam, Soneela, Suryana, Mona, Chan, Lesley Y., Moe, Aung Aung Kywe, Teo, Benjamin K. K., Law, Jaslyn B. K., Sheetz, Michael P., Low, Hong Yee and Yim, Evelyn K. F. (2013) Substrate topography and size determine the fate of human embryonic stem cells to neuronal or glial lineage. Acta Biomaterialia, 9 1: 4535-4545. doi:10.1016/j.actbio.2012.08.018


Author Ankam, Soneela
Suryana, Mona
Chan, Lesley Y.
Moe, Aung Aung Kywe
Teo, Benjamin K. K.
Law, Jaslyn B. K.
Sheetz, Michael P.
Low, Hong Yee
Yim, Evelyn K. F.
Title Substrate topography and size determine the fate of human embryonic stem cells to neuronal or glial lineage
Journal name Acta Biomaterialia   Check publisher's open access policy
ISSN 1742-7061
1878-7568
Publication date 2013-01-01
Sub-type Article (original research)
DOI 10.1016/j.actbio.2012.08.018
Open Access Status Not yet assessed
Volume 9
Issue 1
Start page 4535
End page 4545
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Efficient derivation of neural cells from human embryonic stem cells (hESCs) remains an unmet need for the treatment of neurological disorders. The limiting factors for current methods include being labor-intensive, time-consuming and expensive. In this study, we hypothesize that the substrate topography, with optimal geometry and dimension, can modulate the neural fate of hESCs and enhance the efficiency of differentiation. A multi-architectural chip (MARC) containing fields of topographies varying in geometry and dimension was developed to facilitate high-throughput analysis of topography-induced neural differentiation in vitro. The hESCs were subjected to “direct differentiation”, in which small clumps of undifferentiated hESCs were cultured directly without going through the stage of embryoid body formation, on the MARC with N2 and B27 supplements for 7 days. The gene and protein expression analysis indicated that the anisotropic patterns like gratings promoted neuronal differentiation of hESCs while the isotropic patterns like pillars and wells promoted the glial differentiation of hESCs. This study showed that optimal combination of topography and biochemical cues could shorten the differentiation period and allowed derivation of neurons bearing longer neurites that were aligned along the grating axis. The MARC platform would enable high-throughput screening of topographical substrates that could maximize the efficiency of neuronal differentiation from pluripotent stem cells.
Keyword Neuronal differentiation
Pluripotent stem cells
High-throughput screening
Multiarchitectural array chip
Nanoimprinting
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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Created: Tue, 11 Apr 2017, 12:20:12 EST by Aung Aung Kywe Moe on behalf of Queensland Brain Institute