Extending neurites sense the depth of the underlying topography during neuronal differentiation and contact guidance

Chua, Jie Shi , Chng, Choon-Peng , Moe, Aung Aung Kywe , Tann, Jason Y. , Goh, Eyleen L. K. , Chiam, Keng-Hwee and Yim, Evelyn K. F. (2014) Extending neurites sense the depth of the underlying topography during neuronal differentiation and contact guidance. Biomaterials, 35 27: 7750-7761. doi:10.1016/j.biomaterials.2014.06.008


Author Chua, Jie Shi
Chng, Choon-Peng
Moe, Aung Aung Kywe
Tann, Jason Y.
Goh, Eyleen L. K.
Chiam, Keng-Hwee
Yim, Evelyn K. F.
Title Extending neurites sense the depth of the underlying topography during neuronal differentiation and contact guidance
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
1878-5905
Publication date 2014-06-19
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2014.06.008
Volume 35
Issue 27
Start page 7750
End page 7761
Total pages 12
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract The topography of the extracellular microenvironment influences cell morphology, provides conduct guidance and directs cell differentiation. Aspect ratio and dimension of topography have been shown to affect cell behaviours, but the ability and mechanism of depth-sensing is not clearly understood. We showed that murine neural progenitor cells (mNPCs) can sense the depth of the micro-gratings. Neurite elongation, alignment and neuronal differentiation were observed to increase with grating depth. We proposed a mechanism for depth-sensing by growing neurites: filopodial adhesion in the growth cones favour elongation but the bending rigidity of the neurite cytoskeleton resists it. Thus, perpendicular extension on deeper grooves is unfavourable as neurites need to bend over a larger angle. A quantitative model was developed and its prediction of neurite growth on gratings fit well with the experimental data. The results indicated that mNPC fate can be directed by appropriately designed patterned surfaces.
Keyword Neural progenitor cells
Nanotopography
Neural tissue engineering
Modelling
Neuronal Differentiation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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Created: Tue, 11 Apr 2017, 12:18:18 EST by Aung Aung Kywe Moe on behalf of Queensland Brain Institute