A ratiometric fluorescence probe for imaging sulfur dioxide derivatives in the mitochondria of living cells

Wang, Yongfei, Meng, Qingtao, Zhang, Run, Jia, Hongmin, Zhang, Xuehu and Zhang, Zhiqiang (2017) A ratiometric fluorescence probe for imaging sulfur dioxide derivatives in the mitochondria of living cells. Organic and Biomolecular Chemistry, 15 13: 2734-2739. doi:10.1039/c6ob02779b


Author Wang, Yongfei
Meng, Qingtao
Zhang, Run
Jia, Hongmin
Zhang, Xuehu
Zhang, Zhiqiang
Title A ratiometric fluorescence probe for imaging sulfur dioxide derivatives in the mitochondria of living cells
Journal name Organic and Biomolecular Chemistry   Check publisher's open access policy
ISSN 1477-0520
1477-0539
Publication date 2017-04-07
Sub-type Article (original research)
DOI 10.1039/c6ob02779b
Open Access Status Not yet assessed
Volume 15
Issue 13
Start page 2734
End page 2739
Total pages 6
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Collection year 2018
Language eng
Formatted abstract
Although sulfur dioxide (SO2) plays an essential role in several physiological processes, monitoring of intracellular SO2 at subcellular levels remains challenging due to the lack of rapid and sensitive methods for its quantification in a 100% aqueous solution. Herein, a new hemicyanine dyes-based fluorescence probe, NBD-Id, was designed and synthesized for the detection of SO2 derivatives in pure aqueous solution and living cells. By virtue of a specific 1,4-addition reaction of SO32-HSO3- and the polymethine chain of hemicyanine, significant changes in the absorption and fluorescence emission spectra were observed in less than 20 seconds. The ratiometric fluorescence (F467/F593) detection of SO2 derivatives was then obtained with high sensitivity (detection limit 3.6 nM). It was noted that NBD-Id has a specific response towards SO2 derivatives without interference from other anions and biomolecules. Intracellular fluorescence imaging indicated that NBD-Id is cell membrane permeable and mainly distributed within the mitochondria. Therefore, ratiometric fluorescence imaging of SO2 derivatives in the mitochondria of MCF-7 cells was successfully demonstrated.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Australian Institute for Bioengineering and Nanotechnology Publications
 
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