Differential neuronal and glial expression of nuclear factor I proteins in the cerebral cortex of adult mice

Chen, Kok-Siong, Harris, Lachlan, Lim, Jonathan W. C. , Harvey, Tracey J. , Piper, Michael, Gronostajski, Richard M. , Richards, Linda J. and Bunt, Jens (2017) Differential neuronal and glial expression of nuclear factor I proteins in the cerebral cortex of adult mice. Journal of Comparative Neurology, . doi:10.1002/cne.24206


Author Chen, Kok-Siong
Harris, Lachlan
Lim, Jonathan W. C.
Harvey, Tracey J.
Piper, Michael
Gronostajski, Richard M.
Richards, Linda J.
Bunt, Jens
Title Differential neuronal and glial expression of nuclear factor I proteins in the cerebral cortex of adult mice
Journal name Journal of Comparative Neurology   Check publisher's open access policy
ISSN 1096-9861
0021-9967
Publication date 2017-03-29
Sub-type Article (original research)
DOI 10.1002/cne.24206
Open Access Status Not yet assessed
Total pages 19
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2018
Language eng
Abstract The nuclear factor I (NFI) family of transcription factors plays an important role in the development of the cerebral cortex in humans and mice. Disruption of nuclear factor IA (NFIA), nuclear factor IB (NFIB), or nuclear factor IX (NFIX) results in abnormal development of the corpus callosum, lateral ventricles, and hippocampus. However, the expression or function of these genes has not been examined in detail in the adult brain, and the cell type-specific expression of NFIA, NFIB, and NFIX is currently unknown. Here, we demonstrate that the expression of each NFI protein shows a distinct laminar pattern in the adult mouse neocortex and that their cell type-specific expression differs depending on the family member. NFIA expression was more frequently observed in astrocytes and oligodendroglia, whereas NFIB expression was predominantly localized to astrocytes and neurons. NFIX expression was most commonly observed in neurons. The NFI proteins were equally distributed within microglia, and the ependymal cells lining the ventricles of the brain expressed all three proteins. In the hippocampus, the NFI proteins were expressed during all stages of neural stem cell differentiation in the dentate gyrus, with higher expression intensity in neuroblast cells as compared to quiescent stem cells and mature granule neurons. These findings suggest that the NFI proteins may play distinct roles in cell lineage specification or maintenance, and establish the basis for further investigation of their function in the adult brain and their emerging role in disease.
Keyword Astrocyte
Cerebral cortex
Cortex
Glia
Hippocampus
Microglia
Neuron
NFIA
NFIB
NFIX
Nuclear factor I
Oligodendrocyte
RRID: AB_10608433
RRID: AB_10712361
RRID: AB_1854421
RRID: AB_1854424
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Brain Institute Publications
School of Biomedical Sciences Publications
 
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