Mammalian diaphanous 1 mediates a pathway for E-cadherin to stabilize epithelial barriers through junctional contractility

Acharya, Bipul R., Wu, Selwin K., Lieu, Zi Zhao, Parton, Robert G., Grill, Stephan W., Bershadsky, Alexander D., Gomez, Guillermo A. and Yap, Alpha S. (2017) Mammalian diaphanous 1 mediates a pathway for E-cadherin to stabilize epithelial barriers through junctional contractility. Cell Reports, 18 12: 2854-2867. doi:10.1016/j.celrep.2017.02.078


Author Acharya, Bipul R.
Wu, Selwin K.
Lieu, Zi Zhao
Parton, Robert G.
Grill, Stephan W.
Bershadsky, Alexander D.
Gomez, Guillermo A.
Yap, Alpha S.
Title Mammalian diaphanous 1 mediates a pathway for E-cadherin to stabilize epithelial barriers through junctional contractility
Journal name Cell Reports   Check publisher's open access policy
ISSN 2211-1247
Publication date 2017-03-21
Year available 2017
Sub-type Article (original research)
DOI 10.1016/j.celrep.2017.02.078
Open Access Status DOI
Volume 18
Issue 12
Start page 2854
End page 2867
Total pages 14
Place of publication New York, NY, United States
Publisher Elsevier
Language eng
Subject 1300 Biochemistry, Genetics and Molecular Biology
Abstract Formins are a diverse class of actin regulators that influence filament dynamics and organization. Several formins have been identified at epithelial adherens junctions, but their functional impact remains incompletely understood. Here, we tested the hypothesis that formins might affect epithelial interactions through junctional contractility. We focused on mDia1, which was recruited to the zonula adherens (ZA) of established Caco-2 monolayers in response to E-cadherin and RhoA. mDia1 was necessary for contractility at the ZA, measured by assays that include a FRET-based sensor that reports molecular-level tension across αE-catenin. This reflected a role in reorganizing F-actin networks to form stable bundles that resisted myosin-induced stress. Finally, we found that the impact of mDia1 ramified beyond adherens junctions to stabilize tight junctions and maintain the epithelial permeability barrier. Therefore, control of tissue barrier function constitutes a pathway for cadherin-based contractility to contribute to the physiology of established epithelia.
Keyword Actomyosin
Epithelial barrier
Formins
MDia1
Tissue mechanics
αE-catenin
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 1044041
1058565
1086857
150101367
281903
DP120104667
Institutional Status UQ

 
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