Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury

Wang, Haolu, Zhang, Run, Bridle, Kim R., Jayachandran, Aparna, Thomas, James A., Zhang, Wenzhu, Yuan, Jingli, Xu, Zhi Ping, Crawford, Darrell H. G., Liang, Xiaowen, Liu, Xin and Roberts, Michael S. (2017) Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury. Scientific Reports, 7 . doi:10.1038/srep45374


Author Wang, Haolu
Zhang, Run
Bridle, Kim R.
Jayachandran, Aparna
Thomas, James A.
Zhang, Wenzhu
Yuan, Jingli
Xu, Zhi Ping
Crawford, Darrell H. G.
Liang, Xiaowen
Liu, Xin
Roberts, Michael S.
Title Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury
Formatted title
Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2017-03-28
Sub-type Article (original research)
DOI 10.1038/srep45374
Open Access Status DOI
Volume 7
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2018
Language eng
Formatted abstract
Oxidative stress reflects an imbalance between reactive oxygen species (ROS) and antioxidants, which has been reported as an early unifying event in the development and progression of various diseases and as a direct and mechanistic indicator of treatment response. However, highly reactive and short-lived nature of ROS and antioxidant limited conventional detection agents, which are influenced by many interfering factors. Here, we present a two-photon sensing platform for in vivo dual imaging of oxidative stress at the single cell-level resolution. This sensing platform consists of three probes, which combine the turn-on fluorescent transition-metal complex with different specific responsive groups for glutathione (GSH), hydrogen peroxide (H2O2) and hypochlorous acid (HOCl). By combining fluorescence intensity imaging and fluorescence lifetime imaging, these probes totally remove any possibility of crosstalk from in vivo environmental or instrumental factors, and enable accurate localization and measurement of the changes in ROS and GSH within the liver. This precedes changes in conventional biochemical and histological assessments in two distinct experimental murine models of liver injury. The ability to monitor real-time cellular oxidative stress with dual-modality imaging has significant implications for high-accurate, spatially configured and quantitative assessment of metabolic status and drug response.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Australian Institute for Bioengineering and Nanotechnology Publications
UQ Diamantina Institute Publications
 
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