The defence-associated transcriptome of hexaploid wheat displays homoeolog expression and induction bias

Powell, Jonathan J. , Fitzgerald, Timothy L. , Stiller, Jiri, Berkman, Paul J. , Gardiner, Donald M. , Manners, John M. , Henry, Robert J. and Kazan, Kemal (2017) The defence-associated transcriptome of hexaploid wheat displays homoeolog expression and induction bias. Plant Biotechnology Journal, 15 4: 533-543. doi:10.1111/pbi.12651


Author Powell, Jonathan J.
Fitzgerald, Timothy L.
Stiller, Jiri
Berkman, Paul J.
Gardiner, Donald M.
Manners, John M.
Henry, Robert J.
Kazan, Kemal
Title The defence-associated transcriptome of hexaploid wheat displays homoeolog expression and induction bias
Journal name Plant Biotechnology Journal   Check publisher's open access policy
ISSN 1467-7652
1467-7644
Publication date 2017-04-01
Sub-type Article (original research)
DOI 10.1111/pbi.12651
Open Access Status DOI
Volume 15
Issue 4
Start page 533
End page 543
Total pages 11
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2018
Language eng
Formatted abstract
Bread wheat (Triticum aestivum L.) is an allopolyploid species containing three ancestral genomes. Therefore, three homoeologous copies exist for the majority of genes in the wheat genome. Whether different homoeologs are differentially expressed (homoeolog expression bias) in response to biotic and abiotic stresses is poorly understood. In this study, we applied a RNA-seq approach to analyse homoeolog-specific global gene expression patterns in wheat during infection by the fungal pathogen Fusarium pseudograminearum, which causes crown rot disease in cereals. To ensure specific detection of homoeologs, we first optimized read alignment methods and validated the results experimentally on genes with known patterns of subgenome-specific expression. Our global analysis identified widespread patterns of differential expression among homoeologs, indicating homoeolog expression bias underpins a large proportion of the wheat transcriptome. In particular, genes differentially expressed in response to Fusarium infection were found to be disproportionately contributed from B and D subgenomes. In addition, we found differences in the degree of responsiveness to pathogen infection among homoeologous genes with B and D homoeologs exhibiting stronger responses to pathogen infection than A genome copies. We call this latter phenomenon as ‘homoeolog induction bias’. Understanding how homoeolog expression and induction biases operate may assist the improvement of biotic stress tolerance in wheat and other polyploid crop species.
Keyword Biotic stress
Homoeolog expression bias
Polyploidy
RNA-seq
Wheat
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Alliance for Agriculture and Food Innovation
 
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