Unraveling microbial structure and diversity of activated sludge in a full-scale simultaneous nitrogen and phosphorus removal plant using metagenomic sequencing

Guo, Jianhua, Ni, Bing-Jie, Han, Xiaoyu, Chen, Xueming, Bond, Philip, Peng, Yongzhen and Yuan, Zhiguo (2017) Unraveling microbial structure and diversity of activated sludge in a full-scale simultaneous nitrogen and phosphorus removal plant using metagenomic sequencing. Enzyme and Microbial Technology, 102 16-25. doi:10.1016/j.enzmictec.2017.03.009


Author Guo, Jianhua
Ni, Bing-Jie
Han, Xiaoyu
Chen, Xueming
Bond, Philip
Peng, Yongzhen
Yuan, Zhiguo
Title Unraveling microbial structure and diversity of activated sludge in a full-scale simultaneous nitrogen and phosphorus removal plant using metagenomic sequencing
Journal name Enzyme and Microbial Technology   Check publisher's open access policy
ISSN 1879-0909
0141-0229
Publication date 2017-07-01
Sub-type Article (original research)
DOI 10.1016/j.enzmictec.2017.03.009
Open Access Status Not yet assessed
Volume 102
Start page 16
End page 25
Total pages 10
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2018
Language eng
Formatted abstract
Activated sludge contains highly complex microbial communities, which play crucial roles in pollutant removal performance in wastewater treatment plants (WWTPs). Metagenomic sequencing was applied to characterize microbial community and functional profiles within activated sludge from a full-scale municipal WWTP carrying out simultaneous nitrogen and phosphorous removal (SNPR). We applied the assembled contigs (N90 of 591 bp) and predicted genes to conduct taxonomic and function annotations, respectively. Results revealed the extraordinary microbial diversity of activated sludge, which included detection of minority populations that are difficult to be explored by traditional molecular methods. Taxonomic analysis indicated that the dominant bacterial phyla were Proteobacteria, Nitrospirae, Bacteroidetes, Actinobacteria and Firmicutes. The abundance of the key organisms involved in nitrogen and phosphorous removal were qualified. Aerobic ammonia-oxidizing bacteria distinctly dominate over ammonia-oxidizing archaea and anaerobic ammonium oxidation bacteria. Various key enzymes involved in the global nitrogen cycle were annotated in the activated sludge. High abundance of the known polyphosphate accumulating organisms was detected (approximately 4.89% of the overall population reads), supporting good phosphorous removal performance. This study provides a comprehensive insight into the community structure and diversity of the SNPR system, and will provide foundation for optimal operation of nutrient removal systems.
Keyword Activated sludge
Bacterial diversity
Biological nitrogen removal
Metagenomic sequencing
Microbial community structure
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Advanced Water Management Centre Publications
 
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