Discovery and characterisation of castlerea virus, a new species of Negevirus isolated in Australia

O'Brien, Caitlin A., McLean, Breeanna J., Colmant, Agathe M. G., Harrison, Jessica J., Hall-Mendelin, Sonja, van den Hurk, Andrew F., Johansen, Cheryl A., Watterson, Daniel, Bielefeldt-Ohmann, Helle, Newton, Natalee D., Schulz, Benjamin L., Hall, Roy A. and Hobson-Peters, Jody (2017) Discovery and characterisation of castlerea virus, a new species of Negevirus isolated in Australia. Evolutionary Bioinformatics, 13 . doi:10.1177/1176934317691269


Author O'Brien, Caitlin A.
McLean, Breeanna J.
Colmant, Agathe M. G.
Harrison, Jessica J.
Hall-Mendelin, Sonja
van den Hurk, Andrew F.
Johansen, Cheryl A.
Watterson, Daniel
Bielefeldt-Ohmann, Helle
Newton, Natalee D.
Schulz, Benjamin L.
Hall, Roy A.
Hobson-Peters, Jody
Title Discovery and characterisation of castlerea virus, a new species of Negevirus isolated in Australia
Formatted title
Discovery and characterisation of castlerea virus, a new species of Negevirus isolated in Australia
Journal name Evolutionary Bioinformatics   Check publisher's open access policy
ISSN 1176-9343
Publication date 2017-02-23
Sub-type Article (original research)
DOI 10.1177/1176934317691269
Open Access Status DOI
Volume 13
Total pages 14
Place of publication London, United Kingdom
Publisher Sage Publications
Collection year 2018
Language eng
Formatted abstract
With advances in sequencing technologies, there has been an increase in the discovery of viruses that do not group with any currently described virus families. The newly described taxon Negevirus encompasses a group of viruses displaying an insect-specific phenotype which have been isolated from multiple host species on numerous continents. Using a broad-spectrum virus screening assay based on the detection of double-stranded RNA and next-generation sequencing, we have detected a novel species of negevirus, from Anopheles, Culex, and Aedes mosquitoes collected in 4 geographically separate regions of Australia. Bioinformatic analysis of the virus, tentatively named Castlerea virus, revealed that it is genetically distinct from previously described negeviruses but clusters in the newly proposed Nelorpivirus clade within this taxon. Analysis of virions confirmed the presence of 2 proteins of 24 and 40 kDa which support previous bioinformatic predictions of negevirus structural proteins.
Keyword Insect-specific virus
Mosquito
Negevirus
Virus discovery
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
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