Microbiopsy engineered for minimally invasive and suture-free sub-millimetre skin sampling

Lin, Lynlee L., Prow, Tarl W., Raphael, Anthony P., Harrold, Robert L., Primiero, Clare A., Ansaldo, Alexander B. and Soyer, H. Peter (2013) Microbiopsy engineered for minimally invasive and suture-free sub-millimetre skin sampling. F1000Research, 2 . doi:10.12688/f1000research.2-120.v2

Author Lin, Lynlee L.
Prow, Tarl W.
Raphael, Anthony P.
Harrold, Robert L.
Primiero, Clare A.
Ansaldo, Alexander B.
Soyer, H. Peter
Title Microbiopsy engineered for minimally invasive and suture-free sub-millimetre skin sampling
Journal name F1000Research   Check publisher's open access policy
ISSN 2046-1402
Publication date 2013-07-01
Sub-type Article (original research)
DOI 10.12688/f1000research.2-120.v2
Open Access Status DOI
Volume 2
Total pages 13
Place of publication London, United Kingdom
Publisher Faculty of 1000
Language eng
Abstract We describe the development of a sub-millimetre skin punch biopsy device for painless and suture-free skin sampling for molecular diagnosis and research. Conventional skin punch biopsies range from 2-4 mm in diameter. Local anaesthesia is required and sutures are usually used to close the wound. Our microbiopsy is 0.50 mm wide and 0.20 mm thick. The microbiopsy device is fabricated from three stacked medical grade stainless steel plates tapered to a point and contains a chamber within the centre plate to collect the skin sample. We observed that the application of this device resulted in a 0.21 ± 0.04 mm wide puncture site in volunteer skin using reflectance confocal microscopy. Histological sections from microbiopsied skin revealed 0.22 ± 0.12 mm wide and 0.26 ± 0.09 mm deep puncture sites. Longitudinal observation in microbiopsied volunteers showed that the wound closed within 1 day and was not visible after 7 days. Reflectance confocal microscope images from these same sites showed the formation of a tiny crust that resolved by 3 weeks and was completely undetectable by the naked eye. The design parameters of the device were optimised for molecular analysis using sampled DNA mass as the primary end point in volunteer studies. Finally, total RNA was characterized. The optimised device extracted 5.9 ± 3.4 ng DNA and 9.0 ± 10.1 ng RNA. We foresee that minimally invasive molecular sampling will play an increasingly significant role in diagnostic dermatology and skin research.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Article number 120

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Medicine Publications
UQ Diamantina Institute Publications
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Created: Thu, 06 Apr 2017, 11:04:15 EST by Dr Anthony Raphael on behalf of Learning and Research Services (UQ Library)