Self-organisation after embryonic kidney dissociation is driven via selective adhesion of ureteric epithelial cells

Lefevre, James G., Chiu, Han S., Combes, Alexander N., Vanslambrouck, Jessica M. , Ju, Ali, Hamilton, Nicholas A. and Little, Melissa H. (2017) Self-organisation after embryonic kidney dissociation is driven via selective adhesion of ureteric epithelial cells. Development, 144 6: 1087-1096. doi:10.1242/dev.140228


Author Lefevre, James G.
Chiu, Han S.
Combes, Alexander N.
Vanslambrouck, Jessica M.
Ju, Ali
Hamilton, Nicholas A.
Little, Melissa H.
Title Self-organisation after embryonic kidney dissociation is driven via selective adhesion of ureteric epithelial cells
Journal name Development   Check publisher's open access policy
ISSN 1477-9129
0950-1991
Publication date 2017-03-01
Sub-type Article (original research)
DOI 10.1242/dev.140228
Open Access Status Not yet assessed
Volume 144
Issue 6
Start page 1087
End page 1096
Total pages 10
Place of publication Cambridge, United Kingdom
Publisher Company of Biologists
Collection year 2018
Language eng
Formatted abstract
Human pluripotent stem cells, after directed differentiation in vitro, can spontaneously generate complex tissues via self-organisation of the component cells. Self-organisation can also reform embryonic organ structure after tissue disruption. It has previously been demonstrated that dissociated embryonic kidneys can recreate component epithelial and mesenchymal relationships sufficient to allow continued kidney morphogenesis. Here, we investigate the timing and underlying mechanisms driving self-organisation after dissociation of the embryonic kidney using time-lapse imaging, high-resolution confocal analyses and mathematical modelling. Organotypic self-organisation sufficient for nephron initiation was observed within a 24 h period. This involved cell movement, with structure emerging after the clustering of ureteric epithelial cells, a process consistent with models of random cell movement with preferential cell adhesion. Ureteric epithelialisation rapidly followed the formation of ureteric cell clusters with the reformation of nephron-forming niches representing a later event. Disruption of P-cadherin interactions was seen to impair this ureteric epithelial cell clustering without affecting epithelial maturation. This understanding could facilitate improved regulation of patterning within organoids and facilitate kidney engineering approaches guided by cell-cell self-organisation.
Keyword Cell adhesion
Morphogenesis
Self-organisation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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