In vivo quantitative visualization of hypochlorous acid in the liver using a novel selective two-photon fluorescent probe

Wang, Haolu, Jayachandran, Aparna, Gravot, Germain, Liang, Xiaowen, Thorling, Camilla A., Zhang, Run, Liu, Xin and Roberts, Michael S. (2017). In vivo quantitative visualization of hypochlorous acid in the liver using a novel selective two-photon fluorescent probe. In: Spie Biophotonics Australasia. SPIE BioPhotonics Australasia Conference, Adelaide,SA, Australia, (). 17 - 19 October 2016. doi:10.1117/12.2242978


Author Wang, Haolu
Jayachandran, Aparna
Gravot, Germain
Liang, Xiaowen
Thorling, Camilla A.
Zhang, Run
Liu, Xin
Roberts, Michael S.
Title of paper In vivo quantitative visualization of hypochlorous acid in the liver using a novel selective two-photon fluorescent probe
Formatted title
In vivo quantitative visualization of hypochlorous acid in the liver using a novel selective two-photon fluorescent probe
Conference name SPIE BioPhotonics Australasia Conference
Conference location Adelaide,SA, Australia
Conference dates 17 - 19 October 2016
Convener SPIE
Proceedings title Spie Biophotonics Australasia   Check publisher's open access policy
Journal name Proceedings of SPIE   Check publisher's open access policy
Place of Publication Bellingham, WA, United States
Publisher S P I E - International Society for Optical Engineering
Publication Year 2017
Sub-type Fully published paper
DOI 10.1117/12.2242978
Open Access Status Not yet assessed
ISBN 9781510604346
ISSN 1996-756X
0277-786X
Volume 10013
Total pages 3
Collection year 2017
Language eng
Abstract/Summary Hypochlorous acid (HOCl) plays a vital role in physiological events and diseases. During hepatic ischemia-reperfusion (I/R) injury, HOCl is generated by neutrophils and diffuses into hepatocytes, causing oxidant stress-mediated injury. Although many probes have been developed to detect HOCl, most were difficult to be distinguished from endogenous fluorophores in intravital imaging and only can be employed under one-photon microscopy. A novel iridium(III) complex-based ferrocene dual-signaling chemosensor (Ir-Fc) was designed and synthesized. Ir-Fc exhibited a strong positive fluorescent response only in the presence of HOCl, whereas negligible fluorescent signals were observed upon the additions of other reactive oxygen/nitrogen species and metal ions. There was a good linear relationship between probe responsive fluorescent intensity and HOCl concentration. Ir-Fc was then intravenously injected into BALB/c mice at the final concentration of 50 μM and the mouse livers were imaged using multiphoton microscopy (MPM). In the I/R liver, reduced autofluorescence was detected by MPM, indicating the hepatocyte necrosis. Remarkable enhancement of red fluorescence was observed in hepatocytes with decreased autofluorescence, indicating the reaction of Ir-Fc with endogenous HOCl molecules. The cellular concentration of HOCl was first calculated based on the intensity of MPM images. No obvious toxic effects were observed in histological examination of major organs after Ir-Fc injection. In summary, Ir-Fc has low cytotoxicity, high specificity to HOCl, and rapid "off-on" fluorescence. It is suitable for dynamic quantitatively monitoring HOCl generation using MPM at the cellular level. This technique can be readily extended to examination of liver diseases and injury.
Keyword Fluorescent probe
Hypochlorous acid
Liver
Multiphoton microscopy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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