Quantitative optical imaging of paracetamol-induced metabolism changes in the liver

Liang, Xiaowen, Wang, Haolu, Liu, Xin and Roberts, Michael (2017). Quantitative optical imaging of paracetamol-induced metabolism changes in the liver. In: SPIE BioPhotonics Australasia. SPIE BioPhotonics Australasia, Adelaide, SA, Australia, (). 17 - 19 October 2016. doi:10.1117/12.2242962

Author Liang, Xiaowen
Wang, Haolu
Liu, Xin
Roberts, Michael
Title of paper Quantitative optical imaging of paracetamol-induced metabolism changes in the liver
Conference name SPIE BioPhotonics Australasia
Conference location Adelaide, SA, Australia
Conference dates 17 - 19 October 2016
Convener SPIE
Proceedings title SPIE BioPhotonics Australasia   Check publisher's open access policy
Journal name Proceedings of SPIE   Check publisher's open access policy
Place of Publication Bellingham, WA, United States
Publisher International Society for Optical Engineering
Publication Year 2017
Sub-type Fully published paper
DOI 10.1117/12.2242962
Open Access Status Not yet assessed
ISBN 9781510604346
ISSN 1996-756X
Volume 10013
Total pages 4
Language eng
Formatted Abstract/Summary
Paracetamol is the most readily available and widely used painkiller. However, its toxicity remains the most common cause of liver injury. The toxicity of paracetamol has been attributing to its toxic metabolite, which depletes cellular glutathione (GSH) stores and reacts within cells to increase oxidative stress, leading to mitochondrial dysfunction and cell necrosis. Multiphoton microscopy (MPM) and fluorescence lifetime imaging (FLIM) can provide quantitative imaging of biological tissues and organs in vivo and allow direct visualization of cellular events, which were used to monitor cellular metabolism in paracetamol-induced toxicity in this study. To better understand mechanisms of paracetamol induced liver injury, the redox ratio of NADH/FAD in liver cells were detected and quantified by MPM imaging to represent the relative rates of glycolysis and oxidative phosphorylation within cells. Compared to normal liver, average fluorescence lifetime of NADH and redox ratio of NADH/FAD in hepatocytes was significantly decreased after paracetamol overdose for 12 and 24 hrs, reflecting impaired metabolic activity. GSH levels of treatment groups were significantly lower than those of normal livers, with gradually decreasing from periportal to centrilobular zonation. This imaging technique has significant implications for investigating metabolic mechanisms of paracetamol toxicity.
Keyword Cellular metabolism
Fluorescence lifetime imaging
Multiphoton microscopy
Optical quantitative imaging
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

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