Endometrial biopsy in Bos indicus beef heifers

Ramirez-Garzon, O., Satake, N., Lyons, R., Hill, J., Holland, M. and Mcgowan, M. (2017) Endometrial biopsy in Bos indicus beef heifers. Reproduction in Domestic Animals, 52 3: 526-528. doi:10.1111/rda.12944


Author Ramirez-Garzon, O.
Satake, N.
Lyons, R.
Hill, J.
Holland, M.
Mcgowan, M.
Title Endometrial biopsy in Bos indicus beef heifers
Formatted title
Endometrial biopsy in Bos indicus beef heifers
Journal name Reproduction in Domestic Animals   Check publisher's open access policy
ISSN 1439-0531
0936-6768
Publication date 2017-06-01
Year available 2017
Sub-type Article (original research)
DOI 10.1111/rda.12944
Open Access Status Not yet assessed
Volume 52
Issue 3
Start page 526
End page 528
Total pages 3
Place of publication Berlin, Germany
Publisher Wiley-Blackwell Verlag GmbH
Language eng
Subject 1305 Biotechnology
1103 Animal Science and Zoology
1310 Endocrinology
Abstract An endometrial biopsy allows for a comprehensive assessment of the uterine environment of a breeding female. Although routine in mares, devices used for endometrial biopsies are impracticable in heifers due to the size and structure of the cervix. This report describes the use of a human bronchoscopy biopsy device (Karl Storz 10366L) for collection of endometrial biopsies in Bos indicus beef heifers. The Storz device is smaller and thinner and enabled the collection of an endometrial biopsy in 86% of heifers (n = 44/51). The biopsied tissue was of good quality and suitable for transcriptomic assessment of the endometrium, with total RNA yield and RNA integrity number (RIN) averaging 1.3 μg (range 0.4–5.3 μg) and 7.4 (range 5.7–8.4), respectively.
Formatted abstract
An endometrial biopsy allows for a comprehensive assessment of the uterine environment of a breeding female. Although routine in mares, devices used for endometrial biopsies are impracticable in heifers due to the size and structure of the cervix. This report describes the use of a human bronchoscopy biopsy device (Karl Storz® 10366L) for collection of endometrial biopsies in Bos indicus beef heifers. The Storz® device is smaller and thinner and enabled the collection of an endometrial biopsy in 86% of heifers (n = 44/51). The biopsied tissue was of good quality and suitable for transcriptomic assessment of the endometrium, with total RNA yield and RNA integrity number (RIN) averaging 1.3 μg (range 0.4–5.3 μg) and 7.4 (range 5.7–8.4), respectively.
Keyword Agriculture, Dairy & Animal Science
Reproductive Biology
Veterinary Sciences
Agriculture
Reproductive Biology
Veterinary Sciences
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 568
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Veterinary Science Publications
 
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