Within-day baseline variation in salivary biomarkers in healthy men

Idris, Firman Prathama, Wan, Yunxia, Zhang, Xi and Punyadeera, Chamindie (2017) Within-day baseline variation in salivary biomarkers in healthy men. OMICS, 21 2: 74-80. doi:10.1089/omi.2016.0168

Author Idris, Firman Prathama
Wan, Yunxia
Zhang, Xi
Punyadeera, Chamindie
Title Within-day baseline variation in salivary biomarkers in healthy men
Journal name OMICS   Check publisher's open access policy
ISSN 1536-2310
Publication date 2017-02-01
Sub-type Article (original research)
DOI 10.1089/omi.2016.0168
Open Access Status Not yet assessed
Volume 21
Issue 2
Start page 74
End page 80
Total pages 7
Place of publication New Rochelle, NY, United States
Publisher Mary Ann Liebert
Language eng
Formatted abstract
Saliva is an easily accessible sample and offers practical and noninvasive biomarker solutions as an alternative to blood and urine-based diagnostics. Saliva contains a plethora of biomolecules such as nucleic acids, hormones, proteins, and electrolytes. On the other hand, little is known on the extent to which the biomolecules in saliva vary over time within a given person. This baseline information is crucial for future development of robust saliva-based diagnostics. We have collected unstimulated whole mouth saliva from 20 healthy young men at four times during the day, including before and after a meal. We measured the salivary cortisol, testosterone, C-reactive protein (CRP), stability of genomic DNA (gDNA) and DNA methylation levels of APC, P16INK4a, and PCQAP in these samples. We found that the salivary CRP, DNA methylation, and CD44 gDNA levels did not vary significantly across four time points (p > 0.05) while the salivary cortisol and testosterone levels significantly varied from the morning collection to the afternoon collection (p < 0.05). Furthermore, salivary cortisol levels were significantly affected by eating (p < 0.05). Our study offers a within-person baseline temporal assessment of several clinically relevant biomolecules and diagnostics, and suggests that salivary cortisol and testosterone levels vary over time in a given day whereas CRP and DNA methylation of tumor suppressor genes and CD44 amplification are stable throughout the day. Future research and clinical applications of salivary biomarkers and diagnostics should take into consideration their temporal variations.
Keyword Biomarkers
Personalized medicine
Diagnostic medicine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

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